响应面分析法优化马兰总黄酮提取工艺研究

目的 应用响应面分析法优化马兰总黄酮的提取工艺。方法 以单因素研究为基础，以总黄酮提取量为响应值，以4因素3水平的响应面分析法优化马兰总黄酮的提取工艺。结果 马兰总黄酮的最优提取工艺条件为：乙醇浓度为40%，液料比为〖DK(〗16.5∶1，〖DK)〗提取时间为2 h，提取次数3次，在该条件下，马兰总黄酮提取量预测值为162.538 mg，实际验证值为156.867 mg，并得出各因素对马兰总黄酮提取量的影响大小依次为乙醇浓度、液料比、提取次数、提取时间。结论 响应面分析法可用于优化马兰总黄酮的提取工艺，实验验证值与模型预测值基本相符。

Optimization of Extraction Technique for Total Flavonoids in Kalimeris indica (L.) Sch-Bip by Response Surface Analysis

Objective To optimize the extraction technique for total flavonoids in Kalimeris indica (L.) Sch-Bip by response surface analysis. Methods Based on the single-factor experiment and with the yield of total flavonoids as the response, a four-factor, three-level response surface analysis was used to optimize the extraction technique for total flavonoids in Kalimeris indica (L.) Sch-Bip. Results The results indicated that in the optimal extraction technique, raw material was treated with 40% ethanol (16.5 times) three times (2 h each time). In this condition, the predicted and experimental yields of total flavonoids in Kalimeris indica (L.) Sch-Bip were 162.538 mg and 156.867 mg, respectively. The most important influencing factor for the yield of total flavonoids was ethanol concentration, followed by solid-liquid ratio, extraction times, and extraction duration. Conclusion Response surface analysis can be used to optimize the extraction technique for total flavonoids in Kalimeris indica (L.) Sch-Bip. The experimental value almost accords with the predicted value.