HPLC测定生地黄浓缩颗粒中梓醇的含量

建立测定中药生地黄浓缩颗粒中有效成分梓醇含量ＨＰＬＣ方法。方法：以Ｓｈｉｍ－ｐａｃｋＣＬＣ－ＯＤＳ－Ｃ１８柱为固定相；０．６％乙腈为流动相，检测波长为２１０ｎｍ。结果：梓醇进样量在２－１０μｇ范围内与峰面积呈线性关系。平均回收率为９９．１％。结论ＨＰＬＣ测定中药生地黄浓缩颗粒中梓醇含量方法简便，结果准确。

DETERMINATION OF CATALPOL IN RADIX REHMANNIAE ENRICHED GRAIN BY HPLC

Objective :The catalpol in radix rehmanniae was determination by HPLC. Methods: Catalpol was separated on a ODSC18 column using water acetonitrile (99.4 0.6 )as mobile phase,and detection at210 nm. Results: There was a good linearity ranging from 2 to 10g of catalpol(Y=11 236.6+3 174 626X,r=0.999 3).The average recovery was 99.1%. Conclusion: The method is constant and have a good concentration.It is good method for determination of catalpol in radix rehmanniae enriched grain.