博落回对大鼠心肌Ca2＋.Mg2＋-ATPase与SDH活性的影响及超微结构观察

目的探讨博落回对心肌作用的机制。方法SD大鼠36只分为2个实验组和1个对照组,每组12只;2个实验组分别按1/6LD50、1/3LD50剂量的博落回水煎液灌胃,对照组以蒸馏水灌胃。各组大鼠处死后,每组10只取心肌组织制成匀浆,检测Ca2＋.Mg2＋-ATPase与SDH（琥珀酸脱氢酶）的活性;另2只大鼠心肌行超微结构观察。结果1/6LD50组大鼠心肌内Ca2＋.Mg2＋-ATPase与SDH活性较对照组有显著性升高（P〈0.05）;1/3 LD50组大鼠心肌内Ca2＋.Mg2＋-ATPase与SDH活性同对照组比较无显著性差异（P〉0.05）,但呈下降趋势。超微结构观察到实验组大鼠心肌细胞发生凋亡及1/3 LD50组大鼠出现心肌排列紊乱、断裂等征象。结论博落回对大鼠心肌内Ca2＋.Mg2＋-ATPase与SDH的活性有影响作用,并能诱导心肌细胞凋亡。

Activity of Ca2+.Mg2+-ATPase and SDH in myocardium of rat induced by macleaya cordata and ultrastructures

Objective To study the mechanism of the rat’s myocardium induced by macleaya cordata. Methods36 SD rats were randomly assigned to 2 experimental groups treated by macleaya cordata decoction with a dose of 1/6 LD50, 1/3 LD50 respectively and a control group treated with distilled water by oral administration. The rats were killed 6 hours after the administration for taking the cardiac muscle to examine the Ca2+.Mg2+-ATPase, SDH while observing the structures. ResoultsIn the group treated by macleaya cordata with dose of 1/6 LD50, the activity of Ca2+.Mg2+-ATPase and SDH were increased significantly.However, the groups treated by macleaya cordata with dose of 1/3 LD50 and distilled water, the activity of Ca2+.Mg2+-ATPase,SDH showed no significant change. The apoptosise in myocardium cells of rat’s can be observed in the experimental groups,while the changes were not seen in control group. ConclusionThe results indicate that the macleaye cordata can induce and improve the apoptosis of myocardium cells in low dose, and these effects may be induced by stimulating the activity of Ca2+.Mg2+-ATPase,SDH in myocardium cells.