家兔死后体内2-氨基噻唑啉-4-羧酸分布研究

目的 建立家兔氰化钾灌胃给药致死动物模型,研究氰化物代谢物2-氨基噻唑啉-4-羧酸(ATCA)在家兔体内的死后分布规律.方法 雄性家兔7只(体重约2.0kg～2.5kg)经口灌胃2LD50(10mg/kg)氰化钾水溶液,观察家兔反应,待家兔呼吸、心跳和反射全部消失后立即对家兔进行解剖取心、肝、脾、肺、肾、脑、睾丸、胃壁...

Postmortem distribution of 2-aminothiazolin-4-carboxylic acid in rabbits

Objective To establish a lethal animal model by intragastric administration of potassium cyanide to study the postmortem distribution of cyanide metabolite 2-aminothiazolin-4-carboxylic acid(ATCA).Methods Seven male rabbits(weight 2.0 kg-2.5 kg)were orally administered with 2 LD50(10 mg/kg)potassium cyanide solution,after the respiration,heartbeat and reflexes of the rabbits disappeared,the rabbits were dissected to obtain tissue samples such as heart,liver,spleen,lung,kidney,brain,testicle,stomach wall and muscle,and the samples of heart blood,glass body fluid and urine were also obtained.All the samples were stored at-80℃.Liquid chromatography mass spectrometry was used to determine the content of cyanide metabolite ATCA in the samples Results After KCN administration,rabbits appeared to have rapid respiratory rate,walking fatigue,seizure like convulsion,mydriasis,muscle relaxation,and disappearance various reflexes,which seemed to be"electric shock like"death.The average concentration of cyano(CN-)in heart blood was 11.81μg/ml after death.The distribution of ATCA in rabbits was as follows:spleen>lung>kidney>liver,brain>testicle>heart blood>heart,stomach wall>glass body fluid>right lower limb muscle,blank blood>urine.Conclusion The distribution of ATCA in rabbits is uneven after death of high dose cyanide poisoning.In addition to blood and heart,the spleen,lung,kidney and liver with high distribution should be used for qualitative and quantitative analysis of ATCA.