| Differex~（TM）与Chelex-100法在混合斑检验中的应用比较 |
| |
| 引用本文： | 马骏,张良宾. Differex~（TM）与Chelex-100法在混合斑检验中的应用比较[J]. 中国法医学杂志, 2011, 26(4): 309-310,326 |
| |
| 作者姓名： | 马骏 张良宾 |
| |
| 作者单位： | 常州市公安局刑警支队,江苏常州,213003 |
| |
| 摘 要： | 目的探讨DifferexTM系统法在法医学性侵犯案件混合斑检验中的应用价值,并与传统Chelex-100法进行比较。方法对日常性侵犯案件中的生物检材分别通过DifferexTM系统法与Chelex-100法检验来比较两种方法的优劣。结果采用Chelex-100法提取时,在所检验样本中仅有床单全部获得单一分型,而采用DifferexTM系统法则只有一条内裤的分型为混合型,其它均为单一分型。应用DifferexTM系统法还可以同时提取女性上皮细胞DNA,成功率为100%。采用Chelex-100法时出现双尖峰的频率也明显超过DifferexTM系统法。结论 DifferexTM系统法在法医学性侵犯案件混合斑检验中比Chelex-100法具有更明显的优势,是一种非常有应用前景的方法。
|
| 关 键 词： | 法医物证学 性侵犯 混合斑 DNA检验 DifferexTM系统 |
The evaluation study of the Differex~(TM) system and Chelex-100 method in DNA extraction of mixture stains |
| |
| MA Jun,ZHANG Liangbin. The evaluation study of the Differex~(TM) system and Chelex-100 method in DNA extraction of mixture stains[J]. Chinese Journal of Forensic Medicine, 2011, 26(4): 309-310,326 |
| |
| Authors: | MA Jun ZHANG Liangbin |
| |
| Affiliation: | MA Jun,ZHANG Liangbin/The attachment of criminal police of Changzhou public security of bureau,Jiangsu Changzhou 213003,China |
| |
| Abstract: | Objective To evaluate the application value of the DifferexTM system in DNA extraction of mixture stains from sexsual assault cases,and compare it with Chelex-100 method.Methods DNA of mixture stain samples from sexsual assault cases was extracted by using the DifferexTM system and Chelex-100 method respectively,and then DNA typing results were compared.Results By using the DifferexTM system,the single typing was obtained from all samples except one pants.With the Chelex-100 method,however,the single typing... |
| |
| Keywords: | forensic biological evidence sexsual assault mixture stains DNA test DifferexTM system |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
