| MAP-2在大鼠脑损伤后表达变化的时间规律性研究 |
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| 引用本文： | 吴旭,杨丽,王保捷,张国华,甄博,孙宏杰,王明,何柏林. MAP-2在大鼠脑损伤后表达变化的时间规律性研究[J]. 中国法医学杂志, 2011, 26(4): 269-272,350 |
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| 作者姓名： | 吴旭 杨丽 王保捷 张国华 甄博 孙宏杰 王明 何柏林 |
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| 作者单位： | 1. 中国医科大学法医学院,辽宁沈阳,110001
2. 鞍山市人民检察院技术处,辽宁鞍山,114004 |
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| 基金项目： | 沈阳市科学技术计划课题(2007371071200-1-00); 鞍山市科技三项费用课题(2004373) |
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| 摘 要： | 目的探讨MAP-2在大鼠脑损伤后表达变化的时间规律性。方法以355.09kPa冲击应力致大鼠局灶性脑损伤后,在不同时间应用原位杂交、免疫组化、免疫组化双染色技术检测MAP-2 mRNA和蛋白表达情况。结果 MAP-2mRNA在对照组及假手术组均有表达,伤后30 min表达增强,伤后2d,达到峰值,并维持高水平表达至伤后7d。MAP-2蛋白在对照组及假手术组均有表达,伤后3h开始呈下降趋势,伤后1d,达到最低值,伤后2d起,表达逐渐恢复,伤后15d,恢复至对照组水平。结论脑损伤后MAP-2 mRNA及蛋白表达具有时间规律,可用于法医学推断脑损伤形成时间。
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| 关 键 词： | 法医病理学 微管相关蛋白-2 脑损伤 大鼠 原位杂交 免疫组织化学 |
An experimental study on expression of MAP-2 protein and mRNA after traumatic brain injury in rats |
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| WU Xu,YANG Li,WANG Baojie,ZHANG Guohua,ZHEN Bo,SUN Hongjie,WANG Ming,HE Bailin. An experimental study on expression of MAP-2 protein and mRNA after traumatic brain injury in rats[J]. Chinese Journal of Forensic Medicine, 2011, 26(4): 269-272,350 |
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| Authors: | WU Xu YANG Li WANG Baojie ZHANG Guohua ZHEN Bo SUN Hongjie WANG Ming HE Bailin |
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| Affiliation: | WU Xu1,YANG Li2,WANG Baojie1,ZHANG Guohua1,ZHEN Bo1,SUN Hongjie1,WANG Ming1,HE Bailin1 /1.China Medical University,School of Forensic Medicine,Shenyang 110001,China,2.Department of Technology,People's Procuratorate of Anshan,Anshan 114004 |
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| Abstract: | Objective To investigate the relationship between the expression of MAP-2 and the time course of traumatic brain injury(TBI).Methods The TBI Model was produced by the impact force of 355.09kPa.Immunocytochemical staining,doublelabeled staining,in situ hybridization and computer image analysis were employed.Results The MAP-2 mRNA and protein positive staining can be observed both in control and sham group.The MAP-2 mRNA positive staining remarkably increased from 30 minutes and reached the maximal level at 2... |
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| Keywords: | forensic pathology MAP-2 traumatic brain injury rat in situ hybridization immunohistochemistry |
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