吗啡依赖鼠成瘾相关脑区腺苷酸环化酶的变化

目的观察吗啡依赖鼠成瘾相关脑区腺苷酸环化酶的变化,探讨成瘾相关脑区腺苷酸环化酶的变化与吗啡依赖机制的关系。方法建立吗啡依赖大鼠模型,应用酶组织化学的方法对吗啡依赖鼠七个相关脑区腺苷酸环化酶的变化进行观察,用图像分析系统测定其灰度值,并进行统计学处理。结果吗啡依赖组脑区腺苷酸环化酶含量升高。结论吗啡成瘾相关七个脑区腺苷酸环化酶含量随吗啡依赖时限的延长有增加的趋势,结果提示吗啡依赖机制与特定脑区腺苷酸环化酶的含量变化有密切关系。进一步揭示AC/cAMP-PKA系统上调与吗啡成瘾机制的关系。

Changes of adenylate cyclase on cerebral regions related to mophine dependence in rats

Objective To observe the changes of adenylate cyclase(AC) on cerebral regions related to morphine dependence in rats and investigate the relationship between the enzymological changes and the mechanism of morphine dependence. Methods The technique of enzyme-histochemistry was used to detect the variations of AC of special seven cerebral regions including frontalis cortex, lenticula, corpus amygdaloideun, substantia nigra, hippocampus, periaqueductal gray and locus coerleus in morphine dependent rats. The enzymological changes were observed by optical microscope. Changes of gray degree of these cerebral regions were also observed by using the image analysis system. Results Compared with those in control group, the contents of AC in morphine dependent groups were increased. Conclusion The contents of AC are increase in those regions. The mechanism of morphine dependence close related to the increasing of AC. The correlation of the mechanism of morphine dependence and up-regulation of AC/cAMP-PKA system is discussed.