Determination of methadone in human hair by radioimmunoassay |
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Authors: | S Balabanova H U Wolf |
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Affiliation: | Department of Forensic Medicine, University of Ulm, Federal Republic of Germany. |
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Abstract: | The concentrations of methadone in human hair were measured. The washed hair was cut in 1-mm pieces approximately, then incubated overnight at 45 degrees C with 0.1 m HCl. The extracts were alcalized by 1 m NaOH and diluted by phosphate buffer, pH 7.4. The methadone concentrations were determined by radioimmunoassay. The method is simple, rapid, and practicable for routine determination. |
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