| 一种制备复制缺陷型重组水疱性口炎病毒新方法的建立 |
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| 引用本文： | 贺番,葛金英,郝永清,仇华吉. 一种制备复制缺陷型重组水疱性口炎病毒新方法的建立[J]. 中国兽医科学, 2010, 40(3) |
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| 作者姓名： | 贺番 葛金英 郝永清 仇华吉 |
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| 作者单位： | 贺番(内蒙古农业大学动物科学与医学学院,内蒙古,呼和浩特,010018;中国农业科学院哈尔滨兽医研究所,兽医生物技术国家重点实验室,黑龙江,哈尔滨,150001);葛金英,仇华吉(中国农业科学院哈尔滨兽医研究所,兽医生物技术国家重点实验室,黑龙江,哈尔滨,150001);郝永清(内蒙古农业大学动物科学与医学学院,内蒙古,呼和浩特,010018) |
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| 基金项目： | 国家重点基础研究发展计划(973)项目 |
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| 摘 要： | 为了提高复制缺陷型重组水疱性口炎病毒(VSV△G*G)的制备效率,对传统制备方法进行了改良,在制备过程中先用VSV△G*G转导细胞1h后,再对其进行转染,24h后收集细胞上清并进行毒价测定。并与用传统方法制备的重组病毒的毒价进行比较,结果表明,用改良方法与传统方法制备的重组病毒具有相同的毒价,但改良方法比传统方法节省了20～48h,同时减少了表达的G蛋白诱导细胞融合所造成的细胞损伤。表明改良方法能够代替传统方法制备出高毒价的复制缺陷型重组病毒。
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| 关 键 词： | 复制缺陷型重组水疱性口炎病毒 转染 转导 病毒毒价 VSV△G~*G |
A modified method for generation of replication-defective vesicular stomatitis virus |
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| HE Fan,,GE Jin-ying,HAO Yong-qing,QIU Hua-ji. A modified method for generation of replication-defective vesicular stomatitis virus[J]. Chinese Veterinary Science, 2010, 40(3) |
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| Authors: | HE Fan GE Jin-ying HAO Yong-qing QIU Hua-ji |
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| Affiliation: | HE Fan1,2,GE Jin-ying2,HAO Yong-qing1,QIU Hua-ji2(1.College of Animal Science , Animal Medicine,Inner Mongolia Agricultural University,Hohhot 010018,China,2.State Key Laboratory of Veterinary Biotechnology,Harbin Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Harbin 150001,China) |
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| Abstract: | To improve the efficiency in generation of VSVGG,the traditional method was modified,BHK21 cells were transduced with VSVGG prior to transfection,and 1 hour later,the cells were transfected to generate new VSVGG.Supernatant was collected and titrated on BHK21 cells 24 hours after transfection.In result,the VSVGG generated by the modified method had the same titer as the recombinant virus generated by the traditional method.The modified method could save 20 to 48 hours and minimize the cell damage before har... |
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| Keywords: | transfection transduction viral titer |
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