| 6个五核苷酸STR基因座荧光复合扩增体系的建立 |
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| 引用本文： | 林宇新,王功跃,黄代新,李小廷,陈慧,杨荣芝,杨庆恩. 6个五核苷酸STR基因座荧光复合扩增体系的建立[J]. 中国法医学杂志, 2009, 24(4): 235-238 |
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| 作者姓名： | 林宇新 王功跃 黄代新 李小廷 陈慧 杨荣芝 杨庆恩 |
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| 作者单位： | 1. 华中科技大学同济医学院法医学系,湖北,武汉,430030
2. 舟山市定海区人民检察院,浙江,舟山,316000 |
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| 摘 要： | 目的 构建6个五核苷酸STR基因座荧光复合扩增体系。方法筛选6个多态性程度较高的五核苷酸STR基因座D10S2325、Penta B、Penta W、PentaX、Penta D和PentaE，按照复合扩增引物设计要求，重新设计引物并标记荧光染料，经反复调整和优化，构建6基因座荧光复合扩增体系，并用该复合扩增体系对239名武汉汉族无关个体进行分型。结果6个五核苷酸STR基因座荧光复合扩增体系分型稳定，可重复性好，与各自相应单基因座分型结果完全一致；累积个人识别率达0．999999988，累积非父排除率达0．998063807。结论本文构建的6个五核苷酸STR基因座荧光复合扩增体系具有很高的法医学实用价值，可作为商品化试剂盒的有效补充。
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| 关 键 词： | 法医物证学 短串联重复序列 五核苷酸重复单位 遗传多态性 复合扩增 |
Development of a 6-locus pentanucleotide STR fluorescent-multiplex PCR for forensic purposes |
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| LIN Yuxin,WANG Gongyue,HUANG Daixin,LI Xiaoting,CHEN Hui,YANG Rongzhi,YANG Qingen. Development of a 6-locus pentanucleotide STR fluorescent-multiplex PCR for forensic purposes[J]. Chinese Journal of Forensic Medicine, 2009, 24(4): 235-238 |
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| Authors: | LIN Yuxin WANG Gongyue HUANG Daixin LI Xiaoting CHEN Hui YANG Rongzhi YANG Qingen |
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| Affiliation: | LIN Yuxin1,WANG Gongyue2,HUANG Daixin1,LI Xiaoting1,CHEN Hui1,YANG Rongzhi1,YANG Qingen1/ 1.Faculty of Forensic Medicine,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China,2.Peoplep's Procuratorate,Dinghai District,Zhoushan City,Zhejiang Province,Zhejiang Zhoushan 316000 |
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| Abstract: | 】Objective To establish a 6-locus pentanucleotide STR fluorescent-multiplex PCR for forensic purposes.Methods Six highly polymorphic pentanucleotide STR loci,D10S2325,Penta B,Penta W,Penta X,Penta D and Penta E,were selected,and a 6-locus fluorescent-multiplex PCR system was developed through redesigning primers,labeling fluorescence dye,and optimizing experiment conditions.Then,239 unrelated individuals from Wuhan Han population were genotyped using the established multiplex PCR system.Results A 6-locus pentanucleotide STR fluorescent-multiplex PCR system was established successfully.The established tying system was robust,and its cumulative power of discrimination and probability of exclusion were 0.999 999 988 and 0.998 063 807,respectively.Conclusion The 6-locus pentanucleotide STR fluorescent-multiplex PCR developed by us is of high value in forensic application,and it can serve as an effective supplementary of commercial kits. |
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| Keywords: | forensic biological evidence short tandem repeats pentanucleotide repeat motif genetic polymorphism multiplex PCR |
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