|
|||||
|
|
| 轮状病毒VP4蛋白与热稳定肠毒素融合基因的克隆及其植物表达载体的构建 | |
| 引用本文： | 任雪艳,陈创夫,孔庆军,包慧芳,祝建波.轮状病毒VP4蛋白与热稳定肠毒素融合基因的克隆及其植物表达载体的构建[J].中国兽医科学,2005,35(2):103-107. |
| 作者姓名： | 任雪艳 陈创夫 孔庆军 包慧芳 祝建波 |
| 作者单位： | 1. 新疆地方与民族高发病实验室,新疆,石河子,832003;石河子大学 生物工程学院,新疆,石河子,832003;石河子大学 动物科技学院,新疆,石河子,832003 2. 新疆地方与民族高发病实验室,新疆,石河子,832003;石河子大学 生物工程学院,新疆,石河子,832003 3. 石河子大学 生物工程学院,新疆,石河子,832003 |
| 基金项目： | 新疆生产建设兵团博士基金项目(兵博02) |
| 摘 要： | 利用DNA重组技术,将用PCR扩增来的 VP4-ST融合基因分别克隆到原核表达载体pThioHisB及植物表达载体 pBin438 中,成功构建了重组表达质粒 pTh-VP4-ST和 pB-VP4-ST。将pTh-VP4-ST进行SDS-AGE分析,结果表明,表达的目的蛋白以包涵体形式存在,大小约 40ku;同时将pB-VP4-ST转化了农杆菌EHA105。 |
| 关 键 词： | 轮状病毒 VP4 产肠毒素性大肠埃希氏菌 植物重组表达质粒 |
| 文章编号： | 1000-6419(2005)02-0103-05 |
| 修稿时间： | 2004年10月22 |
Cloning of VP4-ST gene fused from rotavirus VP4 protein gene with heat-stable toxin gene and construction of recombinant plant expression plasmid pB-VP4-ST |
|
| REN Xue-yan,CHEN Chuang-fu,KONG Qing-jun,BAO Hui-fang,ZHU Jian-bo.Cloning of VP4-ST gene fused from rotavirus VP4 protein gene with heat-stable toxin gene and construction of recombinant plant expression plasmid pB-VP4-ST[J].Veterinary Science in China,2005,35(2):103-107. | |
| Authors: | REN Xue-yan CHEN Chuang-fu KONG Qing-jun BAO Hui-fang ZHU Jian-bo |
| Institution: | REN Xue-yan~ |
| Abstract: | The recombinant expression plasmids (pTh)-VP4-ST and pB-VP4-ST were successfully constructed by cloning the VP4-ST fused gene fragments, which were amplified with a pair of primers by PCR, into a prokaryotic expression vector pThioHis and a plant expression vector pBin438, respectively. The fusion protein VP4-ST was expressed from the prokaryotic expression plasmid pTh-VP4-ST in BL21 by inducing with IPTG. The SDS-PAGE analysis showed that the expressed fusion protein is 40 ku in size. In addition, the plant expression plasmid pB-VP4-ST was transformed into the Agrobacterium EHA105. |
| Keywords: | rotavirus VP4 enterotoxigenic Escherichia coli plant expression plasmid |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |