Autosomal short tandem repeat analysis of ancient DNA by coupled use of mini- and conventional STR kits |
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Authors: | Oh Chang Seok Lee Sang Jun Park Jun Bum Lee Soong Deok Seo Seung Bum Kim Hye Yeon Kim Jaehyup Kim Yi-Suk Shin Dong Hoon |
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Affiliation: | Institute of Forensic Medicine, Seoul National University College of Medicine, 28, Yongon-Dong, Chongno-Gu, Seoul 110-799, South Korea. |
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Abstract: | Abstract: Multiplex autosomal short tandem repeat (STR) genotyping enables researchers to obtain genetic information from ancient human samples. In this study, we tested newly developed AmpF?STR® MiniFiler? kit for autosomal STR analysis of ancient DNA (aDNA), using human femurs (n = 8) collected from medieval Korean tombs. After extracting aDNA from the bones, autosomal STR analyses were repeated for each sample using the AmpF?STR® MiniFiler? and Identifiler? kits. Whereas only 21.87% of larger‐sized loci profiles could be obtained with the Identifiler? kit, 75% of the same loci profiles were determined by MiniFiler? kit analysis. This very successful amplification of large‐sized STR markers from highly degraded aDNA suggests that the MiniFiler? kit could be a useful complement to conventional STR kit analysis of ancient samples. |
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Keywords: | forensic science autosomal short tandem repeat genotyping medieval human sample AmpFℓSTR® MiniFiler™ kit AmpFℓSTR® Identifiler™ kit ancient DNA Korea |
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