羔羊丘脑下部催产素的免疫组化定位

采用免疫组织化学技术中的链霉素抗生物素 过氧化酶(streptaridin peroxidase,SP)法,对羔羊丘脑下部催产素(oxytocin,OT)神经元的定位及分布进行了研究。结果表明,丘脑下部分泌OT的神经元主要分布在室旁核和视上核,在环核、视上弥散核、弓状核、室周核、穹窿周核、丘脑下部前区、丘脑下部外侧区、丘脑下部后核和乳头体各核团等也有一定数量的阳性神经元;阳性神经纤维仅见于室旁核、视上弥散核、视上核和交叉上核,在正中隆起第三脑室附近有较多数量的阳性神经纤维。观察结果提示,OT在羔羊丘脑下部分布广泛,且丘脑下部OT经神经垂体、第三脑室、血管3条途径释放,经血液循环最终作用于靶细胞而发挥生理作用。

Immunohistochemical localization of oxytocin in hypothalamus of lamb

The immunohistochemical SP method was used to study the distribution of oxytocin in the hypothalamus of the lamb. The oxytocin-immunohistochemical (OT-IR) neurons mainly distributed over nucleus pareventricularis and nucleus supra-optious. In addition, OT-IR neurons were also found in nucleus circularis, nucleus supra-opticus diffuses, nucleus arcuatus, nucleus periventricularis, nucleus perifornicalis, area anterior hypothalami, area lateralis hypothalami, nucleus posterior hypothalami and nucleus mamilaris. OT-IR nerve fibers were only observed in nucleus paraventricularis, nucleus supra-opticus (diffuses), nucleus supra-optious, nucleus suprachiasmaricus and so on. Also, some nerve fibers were found in the medium eminence and the surrounding area of the third ventricle. These results suggested that the distribution of OT in the hypothalamus of dairy goat is extensive and the physiological effection is on target cells by blood circle after releasing by three channels which are the third ventriculus(cerebrospinal fluid),neurohypophysis and blood vessel.