| DNA遗传标记STR基因座的高分辨电泳分型 |
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| 引用本文： | 侯一平,苟清,吴梅筠. DNA遗传标记STR基因座的高分辨电泳分型[J]. 中国法医学杂志, 1996, 0(2) |
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| 作者姓名： | 侯一平 苟清 吴梅筠 |
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| 作者单位： | 华西医科大学法医学系!成都，610041，华西医科大学法医学系!成都，610041，华西医科大学法医学系!成都，610041 |
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| 基金项目： | 卫生部优秀青年科技人才专项科研基金 |
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| 摘 要： | 应用聚丙烯酰胺凝胶水平不连续电泳和自动激光荧光DNA分析等两种高分辨电泳技术，比较和评估它们对4个STR基因座分型的准确性。结果表明，两者对HUMTH01、HUMVWA和HUM-FES三个基因座的分型结果完全一致。对HUMTHF13A基因座分型，聚丙烯酰胺凝胶水平不连续电泳获得的结果与自动激光荧光DNA分析不具可比性。
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| 关 键 词： | 短串联重复序列 自动激光荧光DNA序列分析仪 聚丙烯酰胺凝胶水平不连续电泳 |
COMPARATIVE STUDIES OF TWO METHODS OF ELECTROPHORESIS IN ANALYSIS OF STR LOCI |
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| Hou Yiping,et al.. COMPARATIVE STUDIES OF TWO METHODS OF ELECTROPHORESIS IN ANALYSIS OF STR LOCI[J]. Chinese Journal of Forensic Medicine, 1996, 0(2) |
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| Authors: | Hou Yiping et al. |
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| Abstract: | The fragment polymorphisms of four human short tandem repeat loci (HUMTH01,HUMVWA.HUMFES and HUMTHF13A) were studied by automatic laser fluorescent DNA Sequencer and disPAGE followed by silver staining. The applications of these two techniques in the research of STR locwere evaluated. The results showed that the genotypes of HUMTH01,HUMVWA and HUMFES locdisclosed by these two techniques were the same. But for HUMTHF13A locus,these two techniques revealed different genotypes for the same individual. This indicated that to some extent the analysis results by these two techniques were not comparable. |
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| Keywords: | Short tandem repeats automatic laser fluorescent DNA Sgquencer Dis-PAGE |
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