| 人类A、B抗原单克隆抗体的研制和初步应用 |
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| 引用本文： | 温佩忠,蒯应松,王香菊,徐秀兰. 人类A、B抗原单克隆抗体的研制和初步应用[J]. 中国法医学杂志, 1992, 0(2) |
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| 作者姓名： | 温佩忠 蒯应松 王香菊 徐秀兰 |
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| 作者单位： | 公安部第二研究所，公安部第二研究所，公安部第二研究所，公安部第二研究所 北京 100038，北京 100038，北京 100038，北京 100038 |
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| 摘 要： | 作者用人类A、B型红细胞及A、B型分泌型唾液免疫8周龄的BALB/C小鼠,用经免疫的脾细胞与SP2/O骨髓瘤细胞融合,用血凝法筛选出分泌抗A抗体和抗B抗体的细胞株各3株。经一年多的传代,仍然能稳定地分泌抗A、抗B抗体,抗体特异性专一,培养上清液效价能达到2048倍,并初步应用于血液及体液(斑)的检验。
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| 关 键 词： | ABO血型 单克隆抗体 杂交瘤 效价 |
PREPARATION AND APPLICATION ON MONOCLONAL ANTI-A ANTI-B ANTIBODIES |
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| Wen Peizhong Kuai Yingsong Wang Xiangju Xu Xiulan. PREPARATION AND APPLICATION ON MONOCLONAL ANTI-A ANTI-B ANTIBODIES[J]. Chinese Journal of Forensic Medicine, 1992, 0(2) |
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| Authors: | Wen Peizhong Kuai Yingsong Wang Xiangju Xu Xiulan |
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| Affiliation: | Wen Peizhong Kuai Yingsong Wang Xiangju Xu Xiulan. Institute of Forensic Science,Ministry of Public Security,Beijing 100038 |
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| Abstract: | BALB/C mice were immunized by intraperitoneal injection with A-group erythrocytes, B-group erythrocytes and saliva of A-group,B-group secretor. Immune spleen cells were fused with sp2/0 murine myeloma cells. The supernatants from each well were examined for antibodies by hemagglutination using A-,B-, O-group RBC.3 hybridoma cell lines of secreting anti-A antibodies and 3 hybridoma cell lines of secreting anti-B antibodies were established.These hybridoma cells have maintained and produced antibodies possessing higher specificity and titer.The agglutination titer of supernatants reached 2048-fold. These antibodies were used in the field of forensic science. |
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| Keywords: | ABO blood group Monoclonal antibody Hybridoma Titer |
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