| 应用ELISA检测人IgG进行血痕种属鉴别的研究——酶联免疫在物证检验中的应用研究之一 |
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| 引用本文： | 张琦,陈东风. 应用ELISA检测人IgG进行血痕种属鉴别的研究——酶联免疫在物证检验中的应用研究之一[J]. 中国法医学杂志, 1986, 0(1) |
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| 作者姓名： | 张琦 陈东风 |
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| 作者单位： | 公安部第二研究所，公安部第二研究所 |
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| 摘 要： | 本文应用 ELISA-双抗体夹心法,通过检出血中的人 IgG 鉴定人血痕。双抗体夹心法是常用来检测抗原的一种方法,但在法医学上用于测定血痕种属尚少报道。我们建立的这种方法,新鲜人血痕的阳性结果可测到64万倍。保存三年的陈旧血痕仍可测出。马、牛、羊、狗、猪、鸡、鸭、鸽、兔、驴、骡和鹌鹑均为阴性。由于本法灵敏度高、特异性好、试剂易得,勿须贵重仪器,在物证检验中便于推广。
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| 关 键 词： | 种属鉴别 血痕 人 IgG ELISA |
IDENTIFICATION OF SPECIES OF BLOODSTAINS BY DETECTING HUMAN IgG USING ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA) |
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| Zhang Qi Chen Dongfeng Institute of Forensic Science,Ministry of Public Security,Beijing. IDENTIFICATION OF SPECIES OF BLOODSTAINS BY DETECTING HUMAN IgG USING ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA)[J]. Chinese Journal of Forensic Medicine, 1986, 0(1) |
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| Authors: | Zhang Qi Chen Dongfeng Institute of Forensic Science Ministry of Public Security Beijing |
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| Abstract: | A sandwich method of enzyme-linked immunosorbent assay for identification of species ofbloodstains by detecting the human IgG was reported.Positive reactions were revealed in humanbloodstain extracts 1:640,000 diluted as well as in extracts of 3 years old bloodstains.Therewere no cross reactions between human and animal bloodstains including bloodstains fromcattle,sheep,horse,hog,dog,duck,chicken,pigeon,donkey,mule,quail and rabbit.Thepresent study indicated that this method is superior in specificity,sensitivity and practicality.No expensive instruments are required for performing the test. |
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| Keywords: | Species identification Bloodstains Human IgG Enzyme-Linked Immunosorbent Assay |
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