Typing of human red cell and serum mixtures in three electrophoretic systems. |
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Authors: | P Stringer S K Vintiner L I Stowell D G Thomson |
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Affiliation: | Criminalistics and Forensic Biology Section, Department of Scientific and Industrial Research, Petone, Wellington, New Zealand. |
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Abstract: | The isoenzymes erythrocyte acid phosphatase and phosphoglucomutase were typed in mixed red cell samples which had been derived from two individuals; the protein group specific component was typed in mixed serum samples. Typing was performed by isoelectric focusing on ultrathin polyacrylamide gels. Depending upon the mixture, from 2 to 20% (but typically 5-10%) by volume of a second blood or serum needed to be present in a mixture before it could be detected. In the majority of cases when there was significant mixing, samples were readily identified as a mixture when the results consisted of unusual band patterns or unusual band intensities. There would be a few instances when blood or serum could not be identified as a mixture when masking effects occurred or when the mixture produced a combined, apparently normal, pattern. |
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