| 超高效液相色谱串联质谱法检测血液中的林可霉素 |
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| 引用本文： | 黄健,张云峰,常靖,王炯,于忠山. 超高效液相色谱串联质谱法检测血液中的林可霉素[J]. 刑事技术, 2013, 0(3): 30-32 |
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| 作者姓名： | 黄健 张云峰 常靖 王炯 于忠山 |
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| 作者单位： | 1. 中国人民公安大学,北京,100038
2. 公安部物证鉴定中心,北京,100038 |
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| 基金项目： | 中央级公益性科研院所基本科研业务费专项基金 |
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| 摘 要： | 目的建立超高效液相色谱串联质谱法(UPLC-MS/MS)测定全血中林可霉素的方法。方法样品血使用3mL水提取,涡旋离心,上清液过HLB柱,用10%甲醇溶液淋洗,甲醇洗脱,洗脱液用于UPLC-MS/MS分析。采用ACQUITY UPLC○R HSS T3色谱柱分离,采用电喷雾多反应监测模式(MRM)检测。结果以林可霉素母离子406.987(m/z)和子离子126.011及359.009(m/z)定性、定量。加标回收率在104.98%～120.74%。在S/N≥3的情况下,最低检出限为55.4pg/mL。结论本方法分析速度快,灵敏度高,准确度高,重现性好,可在法庭科学应用推广。
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| 关 键 词： | 林可霉素 UPLC-MS/MS 血液 固相萃取 |
Determination of lincomycin in blood with UPLC-MS/MS |
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| Affiliation: | HUANG Jian, ZHANG Yun-feng, CHANG Jing, et al. (Chinese People＇s Public Security University, Beijing 100038, China) |
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| Abstract: | Objective To study an analytical method for determination of lincomycin in blood. Methods The samples were ex- tracted by SPE and then analyzed by HPLC with ACQUITY UPLC HSS T3 column and electron spray ionization. Results Average re- covery rate of lincomycin ranged from 104.98 to 120.74%. Based on a signal-to-noise ratio of 3 ： 1, the detection limit was 55.4 pg/ ml. Conclusion This method is sensitive, accurate and can be used in casework. |
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| Keywords: | lincomycin UPLC-MS/MS blood solid-phase extraction |
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