| 堆形艾美球虫广东株Ea1A基因的扩增与序列测定 |
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| 引用本文： | 吴德铭,于康震,曹永长,马静云,毕英佐. 堆形艾美球虫广东株Ea1A基因的扩增与序列测定[J]. 中国兽医科学, 2004, 34(8): 44-47 |
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| 作者姓名： | 吴德铭 于康震 曹永长 马静云 毕英佐 |
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| 作者单位： | 1. 华南农业大学,动物科学学院,基因工程研究室,广东,广州,510642
2. 全国畜牧兽医总站,北京,100026 |
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| 摘 要： | 根据GenBank上登录的堆形艾美球虫Ea1A基因序列 ,设计了 1对引物 ,以抽提的广东株的总RNA为模板 ,利用反转录 聚合酶链反应 (RT PCR)扩增获得了Ea1A基因部分片段 ,并将此片段克隆至pGEM T载体中 ,经PCR、限制性内切酶分析和克隆片段序列测定、比较 ,证实了克隆片段的可靠性
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| 关 键 词： | 堆形艾美球虫 Ea1A基因 RT-PCR |
| 文章编号： | 1000-6419(2004)08-0044-04 |
| 修稿时间： | 2004-04-07 |
Amplification,cloning and sequencing of Ea1A gene of Eimeria acervulina isolated from Guangdong Province |
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| WU De-ming. Amplification,cloning and sequencing of Ea1A gene of Eimeria acervulina isolated from Guangdong Province[J]. Chinese Veterinary Science, 2004, 34(8): 44-47 |
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| Authors: | WU De-ming |
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| Affiliation: | WU De-ming~ |
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| Abstract: | Based on Ea1A nucleotide sequence of E.acervulina published, a pair of primers were (designed). Using the total RNA of the strain Guangdong of E.acervulina, which was isolated from an outbreak in Guangdong Province, as a template, a partial segment of Ea1A was amplified by RT-PCR. The Ea1A gene fragment was cloned into pGEM-T Easy vector, and the recombinant plasmid was named pGEM-Ea1A. Finally, the plasmid was identified by PCR, restriction enzyme analysis and sequencing. |
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| Keywords: | Eimeria acervulina Ea1A gene RT-PCR |
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