| 运用二代测序技术拆分混合STR分型 |
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| 引用本文： | 吴玉剑,方慧,刘翠兰,韦甜. 运用二代测序技术拆分混合STR分型[J]. 刑事技术, 2021, 0(1) |
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| 作者姓名： | 吴玉剑 方慧 刘翠兰 韦甜 |
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| 作者单位： | 武汉公安司法鉴定中心;赛默飞世尔科技〔中国〕有限公司 |
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| 摘 要： | 目的探讨二代测序技术在混合STR分型拆分中的应用。方法对一例强制猥亵妇女案中受害人颈部的拭子和血样作DNA提取,以Precision ID GlobalFiler^TM NGS STR Panel v2试剂盒制备文库,经Ion S5测序仪测序,运用Torrent_Suite_v5.2.1软件进行数据分析后,将检测基因座的序列多态STR分型与长度多态STR分型进行比较。结果在D8S1179、D21S11、D2S441、D2S1338、D10S1248五个基因座发现存在序列特异的等位基因亚型,利用这些亚型对混合STR分型进行了成功拆分。结论二代测序技术提供的等位基因序列信息可对混合STR分型的拆分起到帮助作用。
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| 关 键 词： | 法医遗传学 二代测序 混合DNA 短串联重复 序列多态性 |
Separation of Mixed STR Profi les through Next Generation Sequencing:Case Report |
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| WU Yujian,FANG Hui,LIU Cuilan,WEI Tian. Separation of Mixed STR Profi les through Next Generation Sequencing:Case Report[J]. Forensic Science and Technology, 2021, 0(1) |
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| Authors: | WU Yujian FANG Hui LIU Cuilan WEI Tian |
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| Affiliation: | (Judicial Forensic Center of Wuhan Public Security Bureau,Wuhan 430024,China;Thermo Fisher Scientifi c Technology(China)Co.,Ltd,Shanghai 200053,China) |
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| Abstract: | Objective To explore the applicability of next generation sequencing(NGS) into STR(short tandem repeats) genotyping of mixed DNA. Methods DNA was extracted from one sex-harassed woman with both the swab of wiping the woman’s neck and blood samples that were collected through the case. The Precision ID GlobalFilerTM NGS STR Panel V2 was used to prepare the library that was subsequently sequenced by the Ion S5 sequencer. The data were analyzed by TorrentSuitev5.2.1 software, with the relevant STR profiles being compared between the sequence-and length-based STR ones. Results Sequence-specific allelic subtypes were found in five loci: D8S1179, D21S11, D2S441, D2S1338 and D10S1248, thereby having made the relevant mixed STR profiles successfully separated. Conclusion The allelic sequence information parsed through NGS can assist in separating mixed STR profiles. |
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| Keywords: | forensic genetics next generation sequencing(NGS) mixed DNA STR sequence polymorphism |
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