| 3个Y-STR的复合扩增及其单倍型 |
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| 引用本文： | 刘秋玲,吕德坚,伍新尧. 3个Y-STR的复合扩增及其单倍型[J]. 中国法医学杂志, 2002, 17(4): 201-203 |
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| 作者姓名： | 刘秋玲 吕德坚 伍新尧 |
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| 作者单位： | 中山大学中山医学院法医物证,广东广州,510089 |
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| 摘 要： | 目的 建立复合扩增Y-STR基因座的体系,获得广东汉族人的单倍型频率。方法 复合扩增DYS439、DYS437和DYS434三个基因座,用聚丙烯酰胺凝胶电泳银染法进行基因分型,检测广东汉族327名无关男性个体的单倍型。结果 3个基因座分别检出6个、4个和4个等位基因,共38种单倍型,其单倍型的个体识别率为0.8796。结论 Y-STR基因座复合扩增体系和建立的Y染色体STR数据库,在法医学鉴定中有应用意义。
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| 关 键 词： | 法庭科学 Y染色体 短串联重复(STR) |
| 文章编号： | 1001-5728(2002)04-0201-03 |
| 修稿时间： | 2002-03-01 |
Multiplex amplification and haplotype frequencies of triplex Y specific loci |
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| LIU Qiuling,LU Dejian,WU Xinyao/Faulty of Forensic Medicine,Sun Yat-sen University of Medical Sceiences,Guangzhou. Multiplex amplification and haplotype frequencies of triplex Y specific loci[J]. Chinese Journal of Forensic Medicine, 2002, 17(4): 201-203 |
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| Authors: | LIU Qiuling LU Dejian WU Xinyao/Faulty of Forensic Medicine Sun Yat-sen University of Medical Sceiences Guangzhou |
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| Affiliation: | LIU Qiuling,LU Dejian,WU Xinyao/Faulty of Forensic Medicine,Sun Yat-sen University of Medical Sceiences,Guangzhou 510089 |
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| Abstract: | Objective To establish a multiplexing Y-STR system and study haplotype frequencies of 3 Y-specific loci in Guangdong Han population. Methods DYS439, DYS437 and DYS434 loci were amplified simultaneously by using a triplex system and were analyzed by polyacrylamide gel electrophoresis and silver staining. 327 male unrelated individuals from the Han population in Guangdong were tested to determine the haplotypes frequencies. Results 6, 4, 4 alleles of DYS439, DYS437, and DYS434 coci were found, respectively. 38 different haplotypes were detected. The power of discrimination of this system was 0.8796. Conclusion The multiplex amplified system of these 3 Y-specific loci and their database are useful for forensic practice. |
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| Keywords: | Forensic science Y-chromosome Short tandem repeats (STR) |
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