Recovery of DNA and fingerprints from touched documents |
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| Authors: | Jonathan Sewell Ignacio Quinones Carole Ames Bryan Multaney Stuart Curtis Haj Seeboruth Stephen Moore Barbara Daniel |
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| Affiliation: | aDepartment of Forensic Science and Drug Monitoring, King's College London, Franklin-Wilkins Building, 150 Stamford Street, London, SE1 9NH, UK;bDirectorate of Forensic Services, Metropolitan Police Service, New Scotland Yard, 10 Broadway, London, SW1H 0BG, UK |
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| Abstract: | This study investigated the various factors affecting DNA profiling from DNA recovered from fingerprints deposited on paper before and after fingerprint enhancement treatments. The DNeasy® plant mini kit (QIAGEN®) was found to improve DNA recovery from paper by over 150% compared with the QIAamp® mini kit. A significant decrease in the amount of DNA recovered was observed following treatment with DFO and/or Ninhydrin. This decrease in yield did not have a comparably significant effect on the quality of the SGM Plus™ profiles. Furthermore, this study found that whilst certain paper types, such as newspaper, magazine and filter paper allowed for the good recovery of DNA, common office paper and white card, strongly interfered with the recovery of DNA resulting in poor quality profiles. |
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| Keywords: | Forensic Fingerprints DNA profiling Paper DFO Ninhydrin |
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