用Y-STR单倍型信息指导数据库采样分析

目的分析浙江省绍兴诸暨市（县级市）各镇、村和姓的Y-STR单倍型分布，为Y_STR数据库的采样与应用提供依据。方法采集诸暨市17个镇156村的55个姓氏，且各镇一村有同姓人员10人以上（含）的家族样本5903份男性个体血样。采用YfilerTM复合扩增试剂盒进行17个Y-STR分型，所得数据进行镇（乡／街道）／村／姓氏的组合和镇（乡／街道）／村／姓氏／单倍型组合分布情况统计分析。结果在5903份男性样本中，获得1987种Y-STR单倍型，它们分布于235种镇（乡／街道）／＋-t／姓氏组合，共构成2547种镇（乡／街道）／村／姓氏／单倍型组合。各单倍型对应的“镇／村／姓”组合次数出现从1到18次不等，其中有1686种单倍型对应1种镇一村一姓组合（84．9％），绝大部分的单倍型对应1～2种镇一村一姓组合（95．3％）。各镇／村中同姓人员出现的主流分型大部分为1～2种（90．7％）。在镇／村的同姓人员中出现次要分型的频率平均为18．22％。结论Y-STR数据库在诸暨的采样在家族调查的基础上，应针对次要分型较多的姓增加采样量，减少遗漏风险，获得高质量的YSTR数据库。

An analysis of samples collection for database from Y-STR haplotypes

Objective To deveplope a guidline for samples collection of Y-STR database in Zhuji region of Zhejiang Prov- ince. Methods 5903 male bloodstain samples were collected from 56 surnames that belong to 156 villages in 17 towns of Zhuji ar- ea. The samples were typed using a 17 Y-STRs Y-filer PCR amplification Kit. The distribution of Y-STR haplotype was analyzed according to the different combinations of town-village-surname. Results A total of 1987 different haplotypes were found in 235 combinations of town-village-surname. The number of combinations of town-village-surname-haplotype was 2547. The haplotype numbers occurred in the different combinations of town-village-surname ranged from 1 to 18. 1686 unique combination of town-vil- lage-surname-haplotype was observed. Most of haplotypes（95. 3~//00） belong to 1-2 combinations of town-village-surname. One to two major haplotypes was detected in 90. 7% town-village-surname. The minor haplotypes frequency reached 18. 22~. Conclu- sion The results show that more samples are need to be collected from the surnames which villages have a high ratio of minor haplytpes based on the family infromation. This may be helpful to avoid dropping some hapltypes in Y-STR database of Zhuji re- gion in Zheiiang Province.