刑事照相常用紫外光源照射对DNA检验的影响研究

目的探究刑事照相常用紫外光源照射不同客体上的血指印和汗潜指印后对DNA检验产生的影响。方法分别使用254nm和365nm紫外光源在不同照射距离，不同照射时间下对不同客体表面制备的原血指印、微量血指印和汗潜指印检材进行照射，随后进行DNA定量分析。结果原血指印经紫外照射后对DNA检验结果无显著影响，微量血指印和汗潜指印经紫外照射对后续DNA检验结果认定影响较大。结论紫外光波长越短、功率越大、照射距离越短、照射时间越长，对DNA检验结果的影响越大。

The effects of forensic UV light sources on subsequent DNA analysis

Objective To investigate the effects of forensic UV light sources on the subsequent polymerase chain reaction （PCR）-short tandem repeat （STR） analysis of blood fingerprints and latent fingerprints. Methods Blood fingerprints and latent fingerprints on six different materials were exposed for up to 15 minutes to two different UV light sources, they were subsequently analyzed using a PCR system. Results It was found that UV light had no appreciable effect on subsequent PCR analysis for blood fingerprints but had an effect on subsequent PCR analysis for latent fingerprints. Conclusion The shorter wave and more power- ful of the UV light, the longer time and shorter distance was exposed, the less DNA was obtained.