| 抗人N单克隆抗体的理化特性研究及其初步应用 |
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| 引用本文： | 张建,张秀梅,段薇,郭阳,傅百川,傅锦华. 抗人N单克隆抗体的理化特性研究及其初步应用[J]. 中国法医学杂志, 1987, 0(2) |
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| 作者姓名： | 张建 张秀梅 段薇 郭阳 傅百川 傅锦华 |
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| 作者单位： | 辽宁省基础医学研究所，辽宁省基础医学研究所，辽宁省基础医学研究所，辽宁省基础医学研究所，辽宁省基础医学研究所，辽宁省刑事科学技术研究所 |
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| 摘 要： | 本文报告理化因素对抗人N单克隆抗体(ON_1D_3)活性的影响。P~H及反应温度对ON_1D_3单克隆抗体与人红细胞的血凝反应有显著的影响。较适宜的实验条件为P~H7.0,温度30℃。在此条件下对155例新鲜血液及60例陈旧血痕样品进行MN分型。结果与用市售兔抗N血清测定的结果完全一致,证明在上述条件下,ON_1D_3单克隆抗体可用于临床常规血液分型及法医学上对陈旧血痕MN血型的测定。
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| 关 键 词： | 抗N单克隆抗体 MN血型 理化特征 |
PHYSICOCHEMICAL CHARACTER AND PRELIMINARY APPLICATION OF MOUSE MONOCLONAL ANTI-N |
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| Zhang Jian,Zhang Xiumei,Duan Wei,Guo Yang,Fu Baichuan Basic Medical Institute of Liaoning Province,Shenyang Fu Jinhua Forensic Sciences Institute of Liaoning Province,Shenyang. PHYSICOCHEMICAL CHARACTER AND PRELIMINARY APPLICATION OF MOUSE MONOCLONAL ANTI-N[J]. Chinese Journal of Forensic Medicine, 1987, 0(2) |
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| Authors: | Zhang Jian Zhang Xiumei Duan Wei Guo Yang Fu Baichuan Basic Medical Institute of Liaoning Province Shenyang Fu Jinhua Forensic Sciences Institute of Liaoning Province Shenyang |
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| Abstract: | The effects of physicochemical factors on hemeagglutination reaction of monoclonal anti-N antibody(ON_1D_3)were investigated.Under precise physi- cochemical conditions monoclonal anti-N can be used as blood typing re- agents both in clinical blood test as well as blood stains.It was demonstrated that both the pH and the temperature have an obvious effect on the agglu- tination reaction.The best condition for agglutination reaction is pH7.0, 30℃.Under these conditions ON_1D_3 McAb was used,in parallel with poly- clonal rabbit antisera,for N typing of 155 random blood donors and 60 old blood stains.The results obtained were in perfect agreement |
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| Keywords: | monoclonal anti-N antibodies MN blood type |
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