3种常用PCR扩增试剂盒检验血样DNA的结果比较

目的探讨常用的ProfilerPlusTM、IdentifilerTM与Powerplex(16试剂盒检验血样DNA的差异。方法510名无关中国汉族个体血样,分别用ProfilerPlusTM与Powerplex(16试剂盒进行DNA检验,然后对有不同检验结果的同一样本,再用ProfilerPlusTM、IdentifilerTM与Powerplex(16等三种试剂盒进行检验,并比较其结果。结果在510名个体血样的DNA检测结果中,发现同一样本有不同结果的有7例,其差异率为1.3725%;ProfilerPlusTM、IdentifilerTM与Powerplex(16各有1例在D13S317或FGA基因座上出现等位基因缺失现象,缺失率为0.1961%;ProfilerPlusTM有5例在D8S1179基因座上出现扩增严重不平衡现象,相应的IdentifilerTM与Powerplex(16试剂盒的检验结果为正常杂合子。结论ProfilerPlusTM、IdentifilerTM与Powerplex(16试剂盒检验血样DNA均会出现扩增不平衡和/或基因丢失现象,其发生几率IdentifilerTM与Powerplex(16试剂盒较ProfilerPlusTM少。

Comparison of DNA typing difference on blood samples of the three general PCR reaction kits

Objective To explore the difference of the testing results on blood samples using Profiler Plus TM ,Identifiler TM and Powerplex16 kits. Methods The DNA in 510 blood samples of unrelated individuals of Han population in China were analyzed by Profiler Plus TM and Powerplex16 kits,and then the same samples with variant genotypes of which were again detected by the three different PCR kits forementioned and it was compared to the detecting results of 2 times early or late to the samples.Results 7 samples with different genotypes were observed in 510 individuals,and the odds was 1.3725%; there was severally 1 sample with allele dropout at D13S317 or FGA loci using Profiler Plus TM 、Identifiler TM and Powerplex16 kits,and the probability of allele dropout was 0.1961%. The peak height imbalance at D8S1179 locus were observed in 5 samples in Profiler Plus TM kit,but the types were normal heterozygotes in Identifiler TM and Powerplex16 kits. Conclusion Allele dropout and/or peak height imbalance could happen to test DNA of blood samples using each of the three PCR kits,but the probability in Identifiler and Powerplex16 is less than Profiler Plus TM .