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| 应用自动化工作站提取常见生物样本DNA | |
| 引用本文： | 严江伟,王顺霞,任贺,荆玉婷,高林林,唐晖,焦章平,刘雅诚. 应用自动化工作站提取常见生物样本DNA[J]. 中国法医学杂志, 2007, 22(2): 73-75 |
| 作者姓名： | 严江伟 王顺霞 任贺 荆玉婷 高林林 唐晖 焦章平 刘雅诚 |
| 作者单位： | 1. 北京市公安局法医检验鉴定中心,北京,100085;中国科学院北京基因组研究所,北京,101318 2. 北京市公安局法医检验鉴定中心,北京,100085 3. 北京市人民警察学院,北京,100088;山西医科大学,山西,太原,030001 4. 山西医科大学,山西,太原,030001 5. 杭州市公安局,浙江,杭州,310004 |
| 摘 要： | 目的建立使用自动化工作站提取法医案件生物样本DNA的方法。方法选用Biomek 3000自动化工作站,采用DNA IQTM系统及Chelex法对法医案件中常见生物样本进行DNA提取,荧光定量技术进行定量,PCR扩增16个STR基因座并与手工提取方法比较。结果与手工提取方法进行比较,选用自动化工作站结合使用DNAIQTM系统及Chelex法提取DNA可获得满意的STR检验结果。结论自动化工作站可用于法医案件中常见生物样本的DNA提取。 |
| 关 键 词： | 法医物证学 自动化工作站 DNA提取 定量 STR检验 |
| 文章编号： | 1001-5728(2007)02-0073-03 |
| 修稿时间： | 2006-01-26 |
Study on the application of the automation workstation in DNA extraction of biological samples |
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| YAN Jiang-wei,WANG Shun-xia,REN He,et al.. Study on the application of the automation workstation in DNA extraction of biological samples[J]. Chinese Journal of Forensic Medicine, 2007, 22(2): 73-75 | |
| Authors: | YAN Jiang-wei WANG Shun-xia REN He et al. |
| Affiliation: | YAN Jiang-wei,WANG Shun-xia,REN He,et al./Forensic Medical Examination & Identification Center of Beijing Public Security Bureau,Beijing 10008,China. |
| Abstract: | Objective To study the application of the automation workstation in DNA extraction of forensic casework samples.Methods The Biomek 3000 laboratory automation workstation with the DNA IQTM System and Chelex-100 DNA extraction program was applied to DNA extraction of forensic casework samples.All DNA samples were quantitated by the fluorescent quantitation technique and genotyped subsequently using the Powerplex16 system.These results were compared with those from manual DNA extraction methods.Results The DNA amount and success ratio of STR analysis with the automation workstation were closed to those from manual DNA extraction methods.Conclusion The Biomek 3000 Laboratory Automation Workstation can be applied in DNA extraction of forensic casework samples. |
| Keywords: | Forensic biological evidence Automation workstation DNA extraction Quantitation STR analysis |
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