| 间接酶标抗体染色法检测石蜡切片中鸡肾型传染性支气管炎病毒的研究 |
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| 引用本文： | 许金俊,王宝安,许益民,万洪全,吴力力,朱坤熹. 间接酶标抗体染色法检测石蜡切片中鸡肾型传染性支气管炎病毒的研究[J]. 中国兽医科学, 1999, 0(8) |
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| 作者姓名： | 许金俊 王宝安 许益民 万洪全 吴力力 朱坤熹 |
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| 作者单位： | 扬州大学畜牧兽医学院动物医学系 |
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| 基金项目： | 江苏省教委自然科学基金 |
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| 摘 要： | 以抗鸡肾型传染性支气管炎病毒（ＮＩＢＶ）Ｔ株的鼠源超免疫血清为一级抗体，辣根过氧化物酶（ＨＲＰ）标记的羊抗鼠ＩｇＧ为二级抗体，建立了检测石蜡切片中ＮＩＢＶ抗原的间接酶标抗体染色技术。经过特异性试验和阻断性试验，表明该方法具有高度的敏感性和特异性。应用该法对人工感染ＮＩＢＶＣ９００１株的鸡气管和肾石蜡切片中的病毒抗原进行了检测，结果：气管从感染后０．５～１１ｄ，肾从感染后１～２０ｄ，均检测到病毒抗原，阳性染色主要集中于气管粘膜上皮细胞和肾小管上皮细胞浆内。
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| 关 键 词： | 鸡 肾型传染性支气管炎病毒 石蜡切片 间接酶标抗体染色法 |
Detection of Avian!Nephropathogenic Infectious Bronchitis Virus from Praffin embeded Tissue Sections by Hyperimmune Serum based Indirect Immunoperoxidase Technique |
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| Xu Jinjun Wan Baoan Xu Yiming Wan Hongqu Wu Lili Zhu Kunxi. Detection of Avian!Nephropathogenic Infectious Bronchitis Virus from Praffin embeded Tissue Sections by Hyperimmune Serum based Indirect Immunoperoxidase Technique[J]. Chinese Veterinary Science, 1999, 0(8) |
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| Authors: | Xu Jinjun Wan Baoan Xu Yiming Wan Hongqu Wu Lili Zhu Kunxi |
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| Abstract: | An indirect immunoperoxidase technique based on hyperimmune serum was developed and applied to detect avian nephropathogenic infectious bronchitis virus in paraffin embeded tissue sections. The hyperimmune serum was highly specific to NIBV. With this Procedurs, NIBV could be detected from 12 hours to 11 days in trachea, 24 hours to 20 days in kindey postinfection. Postive staining were mainly observed in the epithelia of tracheal mucosa, epithelia of renal tubules and ureter. |
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| Keywords: | Avian Nephropathogenic infectious bronchitis virus Paraffin embeded tissue sections Indirect immunoperoxidase technique Chinese library class number S 854.43.201 |
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