Characterization of 26 miniSTR loci for improved analysis of degraded DNA samples |
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Authors: | Hill Carolyn R Kline Margaret C Coble Michael D Butler John M |
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Affiliation: | Biochemical Science Division, National Institute of Standards and Technology, Gaithersburg, MD 20899-8311, USA. becky.hill@nist.gov |
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Abstract: | An additional 20 novel mini-short tandem repeat (miniSTR) loci have been developed and characterized beyond the six previously developed by our laboratory for a total of 26 non-CODIS miniSTR markers. These new markers produce short PCR products in the target range of 50-150 base pairs (bp) by moving the primer sequences as close as possible-often directly next to the identified repeat region. These candidate loci were initially screened based on their small amplicon sizes and locations on chromosomes currently unoccupied by the 13 CODIS STR loci or at least 50 Mb away from them on the same chromosome. They were sequenced and evaluated across more than 600 samples, and their population statistics were determined. The heterozygosities of the new loci were compared with those of the 13 CODIS loci and all were found to be comparable. Only five of the new loci had lower values than the CODIS loci; however, all of these were much smaller in size. This data suggests that these 26 miniSTR loci will serve as useful complements to the CODIS loci to aid in the forensic analysis of degraded DNA, as well as missing persons work and parentage testing with limited next-of-kin reference samples. |
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Keywords: | forensic science DNA typing DNA profiling degraded DNA short tandem repeats STR miniSTR D1GATA113E02 D1S1627 D1S1677 D2S441 D2S1776 D3S3053 D3S4529 D4S2364 D4S2408 D5S2500 D6S474 D6S1017 D8S1115 D9S1122 D9S2157 D10S1248 D10S1435 D11S4463 D12ATA63A05 D14S1434 D17S974 D17S1301 D18S853 D20S482 D20S1082 D22S1045 |
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