| 肌红蛋白降解与人体死亡时间的相关性 |
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| 作者姓名： | Bian J Shen YW Zhao ZQ |
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| 作者单位： | 复旦大学上海医学院法医学系,上海,200032 |
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| 摘 要： | 目的检测人体死亡后骨骼肌中肌红蛋白的降解水平,探讨其与死亡时间的关系。方法分别于人体死后0、4、8、12、24、36、48、60、72h取骨骼肌,置于室温25℃,在不同时间点抽提蛋白质,用Western Blot方法检测肌红蛋白,随后利用生物电泳图像分析软件分析。结果肌红蛋白随死后时间逐步降解。结论肌红蛋白降解的检测可用于推断死亡时间。
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| 关 键 词： | 人体组织 肌红蛋白 死亡时间推断 |
| 文章编号： | 1004-5619(2007)02-0090-02 |
| 修稿时间： | 2006年12月4日 |
Relationship between the degradation of myoglobin and postmortem interval |
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| Bian J,Shen YW,Zhao ZQ.Relationship between the degradation of myoglobin and postmortem interval[J].Journal of Forensic Medicine,2007,23(2):90-91. |
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| Authors: | Bian Jie Shen Yi-Wen Zhao Zi-Qin |
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| Institution: | Shanghai Medical College of Fudan University, Shanghai 200031, China. geniusbian@gmail.com |
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| Abstract: | OBJECTIVE: To observe the degradation of myoglobin in skeletal and cardiac muscles after death and to explore its usefulness in estimation of postmortem interval. METHODS: Human skeletal and cardiac muscles were collected at 0, 4, 8, 12, 24, 36, 48, 60, and 72 hours after death and stored at 25 degrees C. The protein was extracted and examined and analyzed by Western blot and imaging technique. RESULTS: Myoglobin showed a continuously postmortem degradation in both skeletal and cardiac muscles. There was no observed difference in degradation between the two tissues. CONCLUSION: Changes in myoglobin degradation could be a useful parameter for estimation of the postmortem interval. |
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| Keywords: | human tissue myoglobin estimating of postmortem interval |
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