实验性脑挫伤后caspase—1表达的研究

目的阐明不同程度及不同时间脑挫伤后caspase-1表达的变化。方法应用免疫组织化学染色、Western-blotting和RT-PCR方法对48例实验性脑挫伤大鼠的脑组织进行了检验,同时以3例非脑挫伤和3例假手术的脑组织做对照。结果脑挫伤后1h caspase-1即有表达,24-48h达高峰,挫伤后两周caspase-1阳性细胞仍维持较高水平。阳性细胞多集中于挫伤区及周围带皮质和脑实质内海马区,不同程度脑挫伤各组之间阳性细胞面积存在差异;损伤组caspase-1酶原裂解增加;损伤组caspase-1mRNA表达量明显高于对照组;不同程度脑挫伤组之间于各个时间段caspase-1mRNA表达未见差异。 结论 Caspase-1表达增加,可辅助诊断1h-14d的脑挫伤,并可根据caspase-1阳性细胞面积的变化来确定脑挫伤程度,同时对判断挫伤时间方面也有一定的价值。caspase-1参与脑挫伤后的神经细胞凋亡。

The study on expression of caspase-1 in experimental brain contusion of different severity in rats

Objective To investigate the expression of caspase-1 in different posttraumatic intervals and severity of brain injury. Methods The study examined brain tissue samples of slight (n=24), severe (n=24) brain injury and control (n=6) of rat, using immunohistochemical staining, western-blot and RT-PCR method. Results Up-regulating of caspase-1 expression was found in tissue from traumatic brain injury compared with controls in early 1 hour after TBI, and lasted for 2 weeks. Caspase-1 cleaved after brain injury. Conclusion These findings are the first time to demonstrate the study of different intense brain injury. The increasing of caspase-1 expression indicates that the TBI exists. The threshold area of caspase-1 positive cells is different in different severity of brain injury.