内毒素对仓鼠肾细胞抗氧化酶活性的影响及阳离子A对其的保护作用

将仓鼠肾细胞(BHK-21)随机分为3组:对照组(Ⅰ组)、内毒素组(Ⅱ组)、内毒素+阳离子A组(Ⅲ组),分别在第3、6、12、24小时收集细胞制备细胞匀浆,采用黄嘌呤氧化酶法检测总超氧化物歧化酶的活性;用比色法定量测定谷胱甘肽-S转移酶、过氧化氢酶、谷胱甘肽过氧化氢酶、谷胱甘肽、总抗氧化能力活性;用考马斯亮蓝法测定细胞匀浆中的蛋白含量,探讨内毒素对体外培养仓鼠肾细胞的抗氧化酶活性的影响以及阳离子A对其的保护作用。结果表明,与Ⅰ组相比,Ⅱ组各种抗氧化酶的活性显著降低(P<0.05);Ⅲ组与Ⅱ组相比,抗氧化酶活性显著增高(P<0.05)。证实内毒素能诱导体外培养的肾细胞的抗氧化酶活性降低,阳离子A能有效保护细胞内的抗氧化酶。

Studies on antioxidant capacity of hamsters rat renal cells induced by endotoxin in vitroand protection of cation A

The cultured hamsters rat renal cells(BHK-21) in vitro were randomly divided into three groups including normal control(GroupⅠ),ET treatment(GroupⅡ),ET+CA treatment(Group Ⅲ),each group included 4 parallel.They were collected and homogenated after cultured for 3,6,12,24 hours,respectively.Total superoxide dismutase activity was detected using the method of xanthine oxidase,and gluta-thione S transferase,catalase,glutathione catalase,glutathione,total antioxidant capacity activity were detected by colorimetric and quantitative method,and homogenate protein content of the cells was detected using the method of Coomassie brilliant blue G-250 to explore antioxidant enzyme activity of endotoxin(ET) to hamster kidney cells in vitro and protection action by cation A(CA).The results showed that all antioxidant enzyme activities of GroupⅡ were significantly lower than GroupⅠ(P<0.05),and antioxidant enzyme activities in GroupⅢ increased significantly compared with GroupⅡ(P<0.05).The present study confirmed that ET could reduce the activity of nephrocyte antioxidant enzymes,and CA could effectively protect antioxidant enzymes in cells.