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| 全血中DNA6种提取方法的比较 | |
| 引用本文： | 畅晶晶,张素华,李莉. 全血中DNA6种提取方法的比较[J]. 法医学杂志, 2009, 25(2): 109-111,114. DOI: 10.3969/j.issn.1004-5619.2009.02.009 |
| 作者姓名： | 畅晶晶 张素华 李莉 |
| 作者单位： | 1. 复旦大学上海医学院法医学系,上海200032;司法部司法鉴定科学技术研究所上海市法医学重点实验室,上海200063 2. 司法部司法鉴定科学技术研究所上海市法医学重点实验室,上海200063;苏州大学医学部法医学系,江苏苏州215123 3. 司法部司法鉴定科学技术研究所上海市法医学重点实验室,上海,200063 |
| 基金项目： | 中央级科研院所社会公益研究专项基金 |
| 摘 要： | 目的 比较经典有机法、改良有机法、常规Chelex-100法、IQ法、Qiagen法及SP法6种方法在提取DNA纯度和得率上的差异.方法 收集10名健康志愿者的静脉全血各5mL,分别采用6种方法提取基因组DNA,通过紫外分光光度仪和荧光定量分析技术检测产物的纯度和浓度,计算得率,并使用统计软件对结果进行分析.结果 常规Chelex-100法所得DNA的纯度明显低于其他方法,而另外5种方法所得DNA纯度的差异不具有统计学意义.改良有机法得率最低,IQ法得率最高.统计结果表明试剂盒方法抽提全血DNA的得率明显高于经典有机法、常规Chelex-100法和改良有机法,其差异具有统计学意义.结论 与有机法和常规Chelex-100法相比,高质量试剂盒类方法更有利于法医学检材的DNA抽提. |
| 关 键 词： | 法医遗传学 提取法 试剂盒,诊断 DNA定量 |
Comparing and Evaluating Six Methods of Extracting Human Genomic DNA from Whole Blood |
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| CHANG Jing-jing,ZHANG Su-hua,LI Li. Comparing and Evaluating Six Methods of Extracting Human Genomic DNA from Whole Blood[J]. Journal of Forensic Medicine, 2009, 25(2): 109-111,114. DOI: 10.3969/j.issn.1004-5619.2009.02.009 | |
| Authors: | CHANG Jing-jing ZHANG Su-hua LI Li |
| Affiliation: | CHANG Jing-jing1,2,ZHANG Su-hua2,3,LI Li2 (1. Department of Forensic Medicine,Shanghai Medical College,Fudan University,Shanghai 200032,Chi- na,2. Shanghai Key Laboratory of Forensic Medicine,Institute of Forensic Science,Ministry of Justice,P. R.China,Shanghai 200063,China,3. Department of Forensic Medicine,Medical College of Soochow Uni- versity,Suzhou 215123,China) |
| Abstract: | Objective Comparing the differences in purity and yield among six methods of extracting human genomic DNA from whole blood, which included Classic Phenol-chloroform extraction, modified combined technique composed of improved Phenol-chloroform extraction and Chelex-100 extraction, Chelex-100 extraction, IQ, Qiagen and SP. Methods Ten samples of intravenous whole blood(5 mL/sample) were collected and human genomic DNA was extracted with these six methods. The purity and concentration of the DNA products were... |
| Keywords: | forensic genetics extraction reagent kits diagnostic DNA quantitative |
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