| PowerPlex~(TM) 16体系在亲子鉴定中的应用评估 |
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| 引用本文： | 孙宏钰,伍新尧,曾艳红,邢浩伟,侯婧,陈国恩. PowerPlex~(TM) 16体系在亲子鉴定中的应用评估[J]. 中国法医学杂志, 2002, 17(4): 207-210 |
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| 作者姓名： | 孙宏钰 伍新尧 曾艳红 邢浩伟 侯婧 陈国恩 |
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| 作者单位： | 中山大学中山医学院法医物证学教研室,广东广州,510089 |
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| 摘 要： | 目的 评估PowerPlexTM16体系在亲子鉴定中的检验能力。方法 以633例亲子鉴定案例为基础,调查PowerPlexTM16体系15个STR基因座的群体遗传学数据资料,并对该体系在亲子鉴定中的排除能力及遗传稳定性进行评估。结果 879名无关个体共检出197个等位基因,739种基因型,累计个体识别力为1×10-30,累计非父排除率为0.999999999999987。633例亲子鉴定案件中有95例确定为排除亲权,平均排除指标为6个。18例表现出1个STR基因座突变的现象,1例表现出2个STR基因座突变的现象。结论 PowerPlexTM16体系应用于亲子鉴定是高效、可靠的。
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| 关 键 词： | 法庭科学 复合扩增 亲子鉴定 |
| 文章编号： | 1001-5728(2002)04-0207-04 |
| 修稿时间： | 2002-03-01 |
The evaluation of PowerPlexTM16 used in paternity testing |
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| SUN Hongyu,WU Xinyao,ZENGYanhong,et al.. The evaluation of PowerPlexTM16 used in paternity testing[J]. Chinese Journal of Forensic Medicine, 2002, 17(4): 207-210 |
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| Authors: | SUN Hongyu WU Xinyao ZENGYanhong et al. |
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| Affiliation: | SUN Hongyu,WU Xinyao,ZENGYanhong,et al. /Department of Forensic Biology,Zhongshan Medical College,Zhongshan University,Guangzhou,510089 |
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| Abstract: | Objective To evaluate the power of the PowerPlex?6 system for paternity testing. Method 633 cases of paternity testing were studied. After the megaplex STR amplification, the PCR products were genotyped by using the ABI377 DNA Sequencer. Results Among the 879 unrelated individuals, 197 alleles and 739 different genotypes were observed. The cumulated discriminating power was 1x 10-30. And the cumulative chance of exclusion was 0.999999999999987. 95 out of 633 cases were excluded. The RCP of all unexcluded cases were ≥0.9990. Gene mutations were found in 19 cases. Conclusion The Po-werPlex?16 system is powerful and reliable for paternity testing. |
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| Keywords: | Forensic science Multiplexing Paternity testing |
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