Automated washing of FTA Card punches and PCR setup for reference samples using a LIMS-controlled Sias Xantus automated liquid handler |
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| Authors: | Michael Stangegaard,Addie N. Olsen,Tobias G. Frø slev,Anders J. Hansen,Niels Morling |
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| Affiliation: | Section of Forensic Genetics, Department of Forensic Medicine, Faculty of Health Sciences, University of Copenhagen, DK-2100 Copenhagen, Denmark |
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| Abstract: | We have implemented and validated automated methods for washing FTA Card punches containing buccal samples and subsequent PCR setup using a Sias Xantus automated liquid handler. The automated methods were controlled by worklists generated by our LabWare Laboratory Information Management System (LIMS). The protocols were validated according to EN/ISO 17025 for use with STR amplifications kits AmpF?STR® Identifiler® and Y-filer® (Applied Biosystems). |
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| Keywords: | Laboratory automation LIMS FTA Reference samples |
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