Automated extraction of DNA and PCR setup using a Tecan Freedom EVO liquid handler |
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| Authors: | Michael Stangegaard,Tobias G. Frø slev,Rune Frank-Hansen,Susan S. Laursen,Mads Jø rgensen,Anders J. Hansen,Niels Morling |
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| Affiliation: | Section of Forensic Genetics, Department of Forensic Medicine, Faculty of Health Sciences, University of Copenhagen, Denmark |
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| Abstract: | We have implemented and validated automated methods for DNA extraction and PCR setup developed for a Tecan Freedom EVO® liquid handler mounted with a Te-MagS™ magnetic separation device. The DNA was extracted using the Qiagen MagAttract® DNA Mini M48 kit. The DNA was amplified using AmpF?STR® Identifiler®, Y-filer® (Applied Biosystems), GenePrint® FFFL and PowerPlex® Y (Promega). The methods were validated for fresh whole blood and blood from deceased according to EN/ISO 17025. |
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| Keywords: | Laboratory automation Nucleic acid extraction Magnetic beads Reference samples |
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