A production system to generate reference genetic profiles from buccal swab cells on FTA cards |
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Authors: | N. Scaramozzino P. Terrenoire L. Baron |
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Affiliation: | aInstitut National de la Police Scientifique, Laboratoire de Lyon, 31 avenue Franklin Roosevelt, 69134 Ecully Cedex, France;bHamilton Life Science Robotics, Via Crush 8, CH-7402 Bonaduz, Switzerland |
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Abstract: | To generate the large quantity of genetic profiles that continuously feeds the French Reference Sample Database (F.N.A.E.G.), a secure and robust process is required. A complete automated production chain has been developed by Hamilton Robotics and the French Police Scientifique in Lyon to produce genetic profiles from buccal swab cells on FTA® cards. The activities have been divided between Pre- and Post-PCR. The samples on FTA® cards are punched directly into PCR plates. The plates are then transferred onto the Hamilton Microlab® STAR liquid handling instruments. Air displacement pipetting coupled with Hamilton's CO-RE Technology allows a highly secure and contamination free pipetting for FTA® wash and STR® Identifiler® PCR reaction set-up. Plates are sealed and transferred to the Post-PCR system for pooling into 384 format and denaturation. Throughout the process steps a complete sample tracking is performed and sample information is transferred to the LIMS system. This poster describes in detail this production system with an actual throughput of 40,000 samples/month, which is in production since 2006. |
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Keywords: | Automation Forensics Reference Database Genotyping Robotics FTA Casework High-throughput |
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