| 利用COI序列序列鉴定常见嗜尸性蝇种 |
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| 引用本文： | 李进华,蔡继峰,郭亚东,陈凤磊,陈瑶清. 利用COI序列序列鉴定常见嗜尸性蝇种[J]. 中国法医学杂志, 2011, 26(6): 443-446 |
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| 作者姓名： | 李进华 蔡继峰 郭亚东 陈凤磊 陈瑶清 |
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| 作者单位： | 1. 中南大学基础医学院法医学系,湖南长沙,410013
2. 湖南公安警官学校,湖南长沙,410008 |
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| 基金项目： | 湖南省自然科学基金资助项目(11JJ5075); 公安部应用创新计划项目(2011YYCXHNST074); 大学生教育创新基金(YC09139,YC10108,YC10118) |
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| 摘 要： | 目的 检测嗜尸性蝇类线粒体DNA(mtDNA)细胞色素氧化酶辅酶Ⅰ(COI)中278bp序列,鉴定蝇科3属5种嗜尸性蝇,解决其形态学种类鉴定的难题,为死亡时间推断提供技术支持.方法 从15省、市(地区)室外草地家兔尸体上采集蝇科3属5种共计18个蝇类成蝇样本,提取mtDNA进行PCR扩增,序列测定且上传GENBANK,...
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| 关 键 词： | 法医昆虫学 线粒体DNA 细胞色素氧化酶辅酶Ⅰ 嗜尸性蝇类 |
The application of COI gene for species identification of sarcosaphagous flies |
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| LI Jinhua,CAI Jifeng,GUO Yadong,CHEN Fenglei,CHEN Yaoqing. The application of COI gene for species identification of sarcosaphagous flies[J]. Chinese Journal of Forensic Medicine, 2011, 26(6): 443-446 |
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| Authors: | LI Jinhua CAI Jifeng GUO Yadong CHEN Fenglei CHEN Yaoqing |
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| Affiliation: | (LI Jinhua1,CAI Jifeng1,GUO Yadong1,CHEN Fenglei,CHEN Yaoqing/1.School of Basic Medical Sciences,Central South University,Changsha 410013,China,2.The Changsha Public Security Bureau in Hunan,Changsha 410008,China) |
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| Abstract: | Objective To solve the problem of morphological identification of Sarcosaphagous flies and to provide technical supports for estimating the postmortem interval(PMI) in forensic practice,the forensically important species of Sarcosaphagous flies were identified through testing a 278 base pairs region of the mitochondrial cytochrome oxidase I(COI) gene.Methods Eighteen fly specimens,including five species of three genera,were collected from the corpses of rabbits from 15 different areas of China.The mtDNA of ... |
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| Keywords: | forensic entomology mtDNA cytochrome oxidase I sarcosaphagous fly |
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