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| 常见嗜尸性昆虫mtDNA提取方法的比较 | |
| 作者姓名： | Chen YQ Guo YD Li MZ Xiong F Li JB Cai JF |
| 作者单位： | 1. 湖南警察学院.湖南长沙410138 2. 中南大学基础医学院法医学系,湖南长沙,410013 3. 长沙市公安局,湖南长沙,410008 |
| 基金项目： | 国家自然科学基金资助项目(30672354); 公安部应用创新计划项目(2011YYCXHNST074); 2007年度湖南省优秀青年骨干教师培养项目(湘教通[2007]214) |
| 摘 要： | 目的 比较十六烷基三甲基溴化铵(cetyl triethyl ammonium bromide,CTAB)法、十二烷基硫酸钠-醋酸钾(sodium dodecyl sulfate-potassium acetate,SDS-KAc)法和十二烷基硫酸钠-蛋白酶K(sodium dodecyl sulfate-protei... |
| 关 键 词： | 法医学 昆虫学 嗜尸性昆虫 DNA 线粒体 |
Comparison of mtDNA extracting methods for common sarcosaphagous insects |
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| Chen YQ,Guo YD,Li MZ,Xiong F,Li JB,Cai JF.Comparison of mtDNA extracting methods for common sarcosaphagous insects[J].Journal of Forensic Medicine,2011,27(4):265-270. | |
| Authors: | Chen Yao-Qing Guo Ya-Dong Li Mao-Zhi Xiong Feng Li Jian-Bo Cai Ji-Feng |
| Institution: | CHEN Yao-qing1,GUO Ya-dong2,LI Mao-zhi3,XIONG Feng2,LI Jian-bo3,CAI Ji-feng2(1.Hunan Police College,Changsha 410138,China,2.Department of Forensic Medicine,School of Basic Medical Science,Central South University,Changsha 410013,3.Changsha Public Security Bureau,Changsha 410008,China) |
| Abstract: | Objective To compare effects of three different methods for mtDNA extraction from common sarcosaphagous insects including cetyl trimethyl ammonium bromide(CTAB) method,sodium dodecyl sulfate-potassium acetate(SDS-KAc) method and sodium dodecyl sulfate-proteinase K(SDS-PK) method.Methods Seventy-two insects from four species Chrysomya megacephala(Fabricius,1784),Eusilpha bicolor(Fairmaire,1896),Paraeutrichopus pecoudi(Mateu,1954),Vespa velutina(Lepeletier,1836)] were collected from the corpses of the rabbit... |
| Keywords: | forensic medicine entomology sarcosaphagous insects DNA mitochondrial |
| 本文献已被 CNKI 维普 万方数据 PubMed 等数据库收录！ | |