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| 甲醛固定石蜡包埋组织DNA提取方法比较 | |
| 引用本文： | 刘德衍,杨锋,袁磊,韦孝铭,于建宪.甲醛固定石蜡包埋组织DNA提取方法比较[J].中国法医学杂志,2011,26(6):465-467. |
| 作者姓名： | 刘德衍 杨锋 袁磊 韦孝铭 于建宪 |
| 作者单位： | 1. 青岛市李沧区人民检察院,山东青岛,266011 2. 临清市公安局,山东临清,252600 3. 聊城市第二人民医院,山东临清,252600 4. 青岛大学医学院附属青岛市市立医院病理科,山东青岛,266011 |
| 摘 要： | 目的比较甲醛固定石蜡包埋尸检组织中三种提取DNA的方法对DNA质量的影响,寻找一种操作简便、污染较少、经济实用的石蜡包埋组织中提取DNA的方法。方法选取经甲醛固定石蜡包埋的心肌组织20例,分别以二甲苯脱蜡-酚氯仿法、改良TES水浴脱蜡-酚氯仿法、试剂盒法提取DNA,进行电泳分析、紫外分光光度计测定A260/A280值及PCR扩增。结果改良TES水浴脱蜡-酚氯仿法抽提DNA法、二甲苯脱蜡-酚氯仿法分别与试剂盒法所提取DNA的A260/A280值相比较,均有显著性意义（P〈0.01）。二甲苯脱蜡-酚氯仿法、改良TES水浴脱蜡-酚氯仿法所提取DNA的A260/A280值相比较,无显著性意义（P〉0.05）。以改良TES水浴脱蜡-酚氯仿法所得DNA为模板,扩增的目的条带亮度与阳性对照相当。结论结果证实改良TES水浴脱蜡-酚氯仿法简便有效,所用试剂价格低廉,是一种经济实用的石蜡包埋组织DNA提取方法。 |
| 关 键 词： | 法医病理学 甲醛 石蜡包埋组织 DNA提取 |
Comparison of three methods for DNA extraction from formaldehyde fixed and paraffin embedded autopsy tissues |
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| LIU Deyan,YANG Feng,YUAN Lei,WEI Xiaoming,YU Jianxian.Comparison of three methods for DNA extraction from formaldehyde fixed and paraffin embedded autopsy tissues[J].Chinese Journal of Forensic Medicine,2011,26(6):465-467. | |
| Authors: | LIU Deyan YANG Feng YUAN Lei WEI Xiaoming YU Jianxian |
| Institution: | (LIU Deyan1,YANG Feng2,YUAN Lei3,WEI Xiaoming4,YU Jianxian4/1.People's Procuratorate of Licang District of Qingdao,Shandong Qingdao 266011,China,2.Linqing Municipal Public Security Bureau,Shandong Linqing 252600,3.Liaocheng Second People's Hospital,4.Department of Pathology,Qingdao University Medical College affiliated Qingdao Municipal Hospital,China) |
| Abstract: | Objective To investigate the influence of three DNA extraction methods for formalin-fixed and paraffin-embedded autopsy tissue on DNA quality,and look for a simple,less pollution,economic and practical method for DNA extraction from paraffin-embedded autopsy tissue.Methods 20 cases of formalin-fixed and paraffin-embedded myocardial tissue were selected and divided into three groups according to the DNA extraction methods including xylenedewaxing and phenol-chloroform,improved TES dewaxing with water-bath an... |
| Keywords: | forensic pathology formaldehyde paraffin-embedded tissue DNA extraction |
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