| 小鼠皮肤切创愈合过程中纤维细胞募集和分化的时间依赖性 |
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| 作者姓名： | Fan YY Guan DW Wang T Liu WW Zheng JL Zhao ZB Yu TS Ma WX |
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| 作者单位： | 1. 中国医科大学法医学院,辽宁沈阳,110001
2. 中国医科大学法医学院,辽宁沈阳110001;中国刑警学院法医学系,辽宁沈阳110854 |
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| 摘 要： | 目的 观察小鼠皮肤切创愈合过程中,纤维细胞募集和分化随时间变化的规律性.方法 应用免疫荧光技术共定位纤维细胞共表达CD45、Ⅰ型前胶原(procollagen Ⅰ)]和肌成纤维细胞共表达CD45、平滑肌肌动蛋白(α-smooth muscle actin,α-SMA)],计数纤维细胞及其分化成的肌成纤维细胞.结果 ...
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| 关 键 词： | 法医病理学 创伤和损伤 皮肤 纤维细胞 肌成纤维细胞 损伤时间 小鼠 |
Time-dependent recruitment and differentiation of fibrocytes in mouse skin wound healing |
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| Fan YY,Guan DW,Wang T,Liu WW,Zheng JL,Zhao ZB,Yu TS,Ma WX.Time-dependent recruitment and differentiation of fibrocytes in mouse skin wound healing[J].Journal of Forensic Medicine,2011,27(4):246-249. |
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| Authors: | Fan Yan-Yan Guan Da-Wei Wang Tao Liu Wei-Wei Zheng Ji-Long Zhao Zhen-Bin Yu Tian-Shui Ma Wen-Xiang |
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| Institution: | FAN Yan-yan1,GUAN Da-wei1,WANG Tao1,LIU Wei-wei1,ZHENG Ji-long1,2,ZHAO Zhen-bin1,YU Tian-shui1,MA Wen-xiang1(1.School of Forensic Medicine,China Medical University,Shenyang 110001,China,2.Department of Forensic Medicine,China Criminal Police University,Shenyang 110854,China) |
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| Abstract: | Objective To investigate the time-dependent recruitment and differentiation of fibrocytes in skin wound healing. Methods Fibrocytes （expressing CD45 and proeollagen ） and myofibroblasts （expressing CD45 and ol-SMA） were co-localized by immunofluorescent staining. The number of fibrocytes and myofi- broblasts was counted at different post-wounding interval. Results At 3 d after injury, fibrocytes started to recruit at the margin of the wounds. At 5 d after injury, myofibroblasts started to appear in new formed granulation tissue. The number of fibrocytes and myofibroblasts peaked at 7 d post-wounding. Conclusion During skin wound healing, myofibroblasts in granulation tissue originated at least partly from fibrocytic differentiation. The time-dependent recruitment and differentiation of fibroeytes may provide new informa- tion for wound age determination. |
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| Keywords: | forensic pathology wounds and injuries skin fibrocyte myofibroblast wound age mice |
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