| 应用mtDNA16S rRNA基因测序鉴定肉产品种属 |
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| 引用本文： | 艾斯卡尔·买买提,马合木提·哈力克,日沙来提·吐尔地,古丽茹合萨·艾海提.应用mtDNA16S rRNA基因测序鉴定肉产品种属[J].中国法医学杂志,2011,26(5):391-392,396. |
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| 作者姓名： | 艾斯卡尔·买买提 马合木提·哈力克 日沙来提·吐尔地 古丽茹合萨·艾海提 |
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| 作者单位： | 新疆大学生命科学与技术学院资源生物研究所,新疆乌鲁木齐,830046 |
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| 基金项目： | 国家自然科学基金项目(31060152); 新疆维吾尔自治区自然科学基金(2010211A02) |
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| 摘 要： | 目的 应用mtDNA 16S rRNA基因测序方法进行肉产品种属鉴定.方法 15份有关部门送检的未知动物肌肉样本,3份市购猪、牛和羊的肌肉为对照样本.常规酚-氯仿法提取模板DNA,分别用通用引物和特异性引物扩增mtDNA的16S rRNA基因片段,产物用1.5％琼脂糖电泳检测,扩增的基因片段由上海生物工程公司进行序列测...
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| 关 键 词： | 法医物证学 16S rRNA基因:种属鉴定 肉产品 |
Species identification of meat products based on mtDNA 16S rRNA gene sequencing |
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| Askar MAMAT,Mahmut HALIK,Risalat TURDI,Gulruhsa AHAT.Species identification of meat products based on mtDNA 16S rRNA gene sequencing[J].Chinese Journal of Forensic Medicine,2011,26(5):391-392,396. |
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| Authors: | Askar MAMAT Mahmut HALIK Risalat TURDI Gulruhsa AHAT |
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| Institution: | (Askar MAMAT,Mahmut HALIK,Risalat TURDI,Gulruhsa AHAT/College of Life Science and Technology,Xinjiang University,Urumqi 830046,China) |
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| Abstract: | Objective Applied mtDNA 16S rRNA gene sequencing method performed species identification of meat products.Methods 15 unknown animal muscle samples were given for inspection by related authorities,pork,beef and lamb were bought and used as a control.Extract DNA by conventional phenol-chloroform method,amplified mtDNA 16S rRNA gene by using the general primer and the specific primers respectively,products were examined by 1.5% agarose gel electrophoresis.Amplified sequences were sequenced by Shanghai Bio-Engi... |
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| Keywords: | forensic biological evidence 16S rRNA gene species identification meat products |
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