| 高效液相色谱-质谱法测定人尿液中乙基葡萄糖醛酸苷 |
| |
| 引用本文： | 李青,于天晓,袁佩,李剑波,丁世家. 高效液相色谱-质谱法测定人尿液中乙基葡萄糖醛酸苷[J]. 中国法医学杂志, 2011, 26(5): 374-376 |
| |
| 作者姓名： | 李青 于天晓 袁佩 李剑波 丁世家 |
| |
| 作者单位： | 1. 重庆医科大学医学检验系临床检验诊断学教育部重点实验室,重庆,400016
2. 重庆医科大学基础医学院法医教研室,重庆,400016 |
| |
| 基金项目： | 重庆市自然科学基金项目(CSTTC-2007BB5314) |
| |
| 摘 要： | 目的建立高效液相色谱-质谱法测定人尿液中乙基葡萄糖醛酸苷。方法取空白人尿液加入EtG-D5内标工作液和冷甲醇（4℃）,经涡旋、离心,取上清液于水浴氮气吹干,以沉淀杂质,采用Agilent Zorbax Bonus-RP色谱柱分离,以乙腈∶0.1%甲酸水溶液（5∶95）为流动相,采用负离子模式质谱检测,在选择离子监测模式下进行定性和定量测定。结果尿液中EtG在50～5 000ng/mL范围内线性关系良好,检出限为18ng/mL,方法回收率为90.2%～101.7%,相对标准偏差小于10%。结论所建方法简便,具有较高的回收率和精密度,适用于尿液中EtG的检测。
|
| 关 键 词： | 法医毒物分析 乙基葡萄糖醛酸苷 高效液相色谱-质谱 尿液 |
Determination of ethyl glucuronide in human urine by high performance liquid hromatography-mass spectrometry |
| |
| LI Qing,YU Tianxiao,YUAN Pei,LI Jianbo,DING Shijia. Determination of ethyl glucuronide in human urine by high performance liquid hromatography-mass spectrometry[J]. Chinese Journal of Forensic Medicine, 2011, 26(5): 374-376 |
| |
| Authors: | LI Qing YU Tianxiao YUAN Pei LI Jianbo DING Shijia |
| |
| Affiliation: | (LI Qing1,YU Tianxiao1,YUAN Pei2,LI Jianbo2,DING Shijia1/1.The Key Laboratory of Laboratory Medical Diagnostics,Ministry of Education,the Faculty of Laboratory Medicine,Chongqing Medical University,Chongqing 400016,China,2.Department of Forensic Medicine Science,College of Basic Medicine,China) |
| |
| Abstract: | Objective To establish a HPLC-MS method for the determination of EtG in human urine.Methods Urine samples were spiked with the internal standard solution and 4mL cold methanol.After agitation and centrifugation,the supernatant was evaporated to dryness under nitrogen.The HPLC separation was performed on an Agilent Zorbax Bonus-RP column with acetonitrile/0.1% formic acid(5/95 v/v) as mobile phase.The identification and quantification were carried out by negative electrospray ionization and selected ion moni... |
| |
| Keywords: | forensic toxicological analysis EtG high performance liquid chromatography-mass spectrometry(HPLC/MS) urine |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
