Results of the 2008 Colombian paternity testing quality control exercise |
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| Authors: | J.J. Builes,D. Aguirre,A. Manrique,Y. Puerto,M.L. Bravo,A. Gaviria,D. Suá rez,D.L. Sá nchez,L. Beltrá n,W. Usaqué n,A. Castillo,C. Pineda,A. Ibarra,A. Carracedo,L. Gusmã o |
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| Affiliation: | aLaboratorio Genes Ltda, Medellín, Colombia;bInstituto de Biología, Universidad de Antioquia, Medellín, Colombia;cIPATIMUP Institute of Pathology and Molecular Immunology, University of Porto, Porto, Portugal;dLaboratorio de Genética Molecular, Cruz Roja Ecuatoriana, Quito, Ecuador;eLaboratorio de Biología Molecular, Fundación Arthur Stanley Gillow, Bogotá, Colombia;fLaboratorio Genética Molecular de Colombia, Bogotá, Colombia;gLaboratorio de Genética Médica, Universidad Tecnológica de Pereira, Pereira, Colombia;hGrupo de Genética de Poblaciones e Identificación, Universidad Nacional de Colombia, Bogotá, Colombia;iLaboratorio de Genetica, Universidad Industrial de Santander, Bucaramanga, Colombia;jLaboratorio de Identificación Humana, Universidad Manuela Beltrán, Bogotá, Colombia;kLaboratorio de Identificación Genética - IdentiGEN, Universidad de Antioquia, Medellín, Colombia;lInstituto de Medicina Legal, Universidad de Santiago de Compostela, A Coruñaa, Spain |
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| Abstract: | The Reference National Laboratory, Genes Ltda, designated by the Commission of Accreditation and Alertness created by Law 721 of 2001 of Republic of Colombia, organized and coordinated the Quality Control Exercise of 2008 for laboratories undertaking paternity and maternity tests with DNA markers. The Quality Control Exercise included both practical and theoretical exercises. For the practical exercise, three blood samples in FTA Classic Card were sent to each participating laboratory to be genotyped for DNA markers using the routine methodologies in their laboratories. For the theoretical exercise, it was asked to the participating laboratories to calculate the partial and final paternity indexes based on two genetic profiles of an alleged biological father and his son. Allele frequencies were made available to the participants, as well as Y chromosome haplotype database. A total of 12 laboratories have participated with data from 57 STRs, including autosomal and sex chromosome markers. Consensus was found in 37 STRs, 21 in autosomes and 16 Y chromosome linked. The rate of reporting errors was 3.1% (concentrated in just one laboratory). The theoretical exercise had consensus. |
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| Keywords: | Paternity testing Collaborative exercise DNA profiling Quality control exercise Colombia |
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