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Increased sensitivity for amplified STR alleles on capillary sequencers with BigDye XTerminator™ |
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| Authors: | Lynn Janssen Manon Jacobs Nancy Vanderheyden Bram Bekaert Wim Van de Voorde Ronny Decorte |
| Institution: | aUZ Leuven, Department of Forensic Medicine, Laboratory of Forensic Genetics and Molecular Archaeology, Kapucijnenvoer 33, B-3000 Leuven, Belgium;bK.U. Leuven, Department of Human Genetics, Campus Gasthuisberg, Herestraat 49, B-3000 Leuven, Belgium |
| Abstract: | Free fluorescent dyes from PCR primers or amplification products can interfere with the interpretation of STR alleles in an electropherograph especially when the profiles have a low signal intensity. These artefacts can be removed by using a simple procedure based on BigDye® XTerminator™. This procedure requires limited amounts of PCR product, allows to do several loadings on a capillary sequencer starting from the same purified PCR product and also increases the sensitivity for detection of less amplified loci. |
| Keywords: | STR profiling Capillary electrophoresis Fluorescent dye artefacts |
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