Sequencing of mitochondrial DNA and the problem of human specificity |
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| Authors: | Rebecca Renneberg,Anke Heinreich,Ben Krause-Kyora,Melanie Rö pke,Jens Tetens,Eva Simeoni,Thorsten Schwark,Nicole von Wurmb-Schwark |
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| Affiliation: | aInstitute of Experimental Medicine, CAU Kiel & University Hospital Schleswig-Holstein, c/o Arnold-Heller-Str. 3, House 28, D-24105 Kiel, Germany;bInstitute of Legal Medicine, CAU Kiel & University Hospital Schleswig-Holstein, Arnold-Heller-Str. 3, House 28, D-24105 Kiel, Germany;cGraduate School Human Development in Landscapes, Christian-Albrechts-University Kiel, Neufeldstraße 10, D-24118 Kiel, Germany;dInstitute of Animal Breeding and Husbandry, Christian-Albrechts-University Kiel, Hermann-Rodewald-Straße 6, D-24098 Kiel, Germany |
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| Abstract: | When analysing trace materials and degraded DNA the issue of human specificity is highly important. Especially when it comes down to the analysis of mitochondrial DNA which is extremely susceptible to contamination authenticity is the main question. Therefore in the presented study mitochondrial primers were tested on their human specificity. In all cases it was possible to amplify DNA of animals with human mt-primers. These unintentional amplifications could only be decreased by choosing austere PCR parameters. The study implies the importance of comprehensive evaluation of primers, chemicals and PCR parameters. |
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| Keywords: | Mitochondrial DNA Human specificity |
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