Construction and application of four fluorescence labeled multiplex typing system for 3 miniSTR loci |
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Authors: | Shujin Li Ning Liu Xue Bai Jianli Gu Zhiping Hou Bin Cong |
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Affiliation: | Hebei Key Laboratory of Forensic Medicine, Department of Forensic Medicine, Hebei Medical University, Shijiazhuang 050017, PR China |
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Abstract: | We constructed a multiplex PCR system for 3 miniSTR loci D20S482, D3S3053, D6S474. This typing system showed high stability and sensitivity (0.05 ng). Population data investigated in 120 healthy unrelated Chinese Han individuals showed higher genetic polymorphism, with the combined power of discrimination and power of exclusion being 0.998 and 0.84. The amplification product length ranged from 88 bp to 127 bp for all three loci. The successful rate of typing highly degraded samples using this miniSTR multiplex PCR system was significantly higher than using identifiler kit, indicating the multiplex set represents a useful tool in Chinese forensic practice, especially for the highly degraded DNA sample. |
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Keywords: | miniSTR Multiplex PCR Degraded sample Chinese Han population |
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