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Analysis of AmpFlSTR MiniFiler™ loci and its forensic application
Authors:Shigeki Nakamura  Chikako Murakami  Kazuho Maeda  Masamune Kobayashi  Wataru Irie  Bunta Wada  Maiko Hayashi  Chizuko Sasaki  Masataka Furukawa  Katsuyoshi Kurihara
Institution:Department of Legal Medicine, Kitasato University School of Medicine, Sagamihara 228-8555, Japan
Abstract:The allele frequencies of eight MiniFiler™ loci have been analyzed in 101 Japanese individuals living in Kanagawa with informed consent by means of ABI 310 Genetic Analyzer. A total of 7 alleles for D13S317, 8 alleles for D7S820, 11 alleles for D2S1338, 11 alleles for D21S11, 5 alleles for D16S539, 14 alleles for D18S51, 8 alleles for CSF1PO, and 13 alleles for FGA were observed. The polymorphic profiles of these MiniFiler™ loci in the present study were essentially the same as those obtained by using the AmpFlSTR® Identifiler® PCR Amplification kit. The combined matching probability of eight MiniFiler™ loci and cumulative probability of paternity exclusion were estimated as 1.97 × 10−10 and 0.9996, respectively. The MiniFiler™ kit was useful for individual identification in forensic analysis.
Keywords:DNA polymorphism  STR  Population study  Multiplex PCR amplification  Japanese population
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