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A novel platform for the modular integration of forensic assay setup and medium- to high-throughput purification of nucleic acids
Authors:Mario Scherer  Thomas Weierstall  Uta Wegener  Lesley Clifford  Mathew Walker  Thomas Schnibbe  Holger Engel
Affiliation:aQIAGEN GmbH, QIAGEN Strasse 1, 40724 Hilden, Germany;bOrchid Cellmark, Abingdon, Oxfordshire, OX14 1YX, United Kingdom
Abstract:With its extraction and assay setup modules, the QIAsymphony® provides a highly flexible solution for processing forensic samples in a medium- to high-throughput scale. We tested the sensitivity of extraction, precision of sample processing, and accuracy of automated assay setup of this integrated system. Results attest to QIAsymphony's ability to isolate DNA from a spectrum of common forensic samples and process these samples without cross-contamination. Furthermore, accurate assay setup for downstream applications, like PCR, make this system highly suited for enhancing laboratory workflow.
Keywords:Automation   DNA purification   Forensic casework   QIAsymphony
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