Fast PCR amplification using AmpFlSTR Identifiler: Second report |
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Authors: | Kazuhiko Tsukada Yuta Harayama Yoshinobu Kurasawa Koichi Kasahara |
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Affiliation: | Department of Forensic Medicine, Criminal Investigation Laboratory, Nagano Pref. Police H.Q., 3916 Nishijo, Matsushiro-Machi, Nagano-shi, Nagano 381-1232, Japan |
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Abstract: | At ISFG2007, an earlier conference, we presented reports on two fast PCR cycling methods using AmpFlSTR Identifiler. In our current study, which involved PCR amplification using AmpliTaq Gold Fast PCR Master Mix, UP, and a standard PCR thermal cycler rather than the Fast PCR thermal cycler, we succeeded in allele typing while reducing PCR running times by half (to approximately 90 min). |
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Keywords: | Fast PCR STR AmpFlSTR Identifiler AmpliTaq Gold Fast PCR Master Mix |
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