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Fast PCR amplification using AmpFlSTR Identifiler: Second report
Authors:Kazuhiko Tsukada  Yuta Harayama  Yoshinobu Kurasawa  Koichi Kasahara
Affiliation:Department of Forensic Medicine, Criminal Investigation Laboratory, Nagano Pref. Police H.Q., 3916 Nishijo, Matsushiro-Machi, Nagano-shi, Nagano 381-1232, Japan
Abstract:At ISFG2007, an earlier conference, we presented reports on two fast PCR cycling methods using AmpFlSTR Identifiler. In our current study, which involved PCR amplification using AmpliTaq Gold Fast PCR Master Mix, UP, and a standard PCR thermal cycler rather than the Fast PCR thermal cycler, we succeeded in allele typing while reducing PCR running times by half (to approximately 90 min).
Keywords:Fast PCR   STR   AmpFlSTR Identifiler   AmpliTaq Gold Fast PCR Master Mix
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